北京中医药大学学报2024，Vol.47Issue(9)：1272-1281,10.DOI:10.3969/j.issn.1006-2157.2024.09.011
黄芩苷调控炎性因子治疗肠道急性移植物抗宿主病的研究
Study on baicalin alleviating the symptoms of intestinal acute graft versus host disease by inhibiting inflammatory factors
摘要
Abstract
Objective To explore the mechanism of baicalin intervention in gastrointestinal acute graft versus host disease (aGVHD). Methods Peripheral blood mononuclear cells from patients with intestinal aGVHD and healthy volunteers were collected for high-throughput sequencing and differential mRNA enrichment analysis. The intersection of the baicalin target and differential mRNA was analyzed. CB6F1 mice were irradiated with 60Co X-rays. After irradiation,a mononuclear cell suspension (1×107 bone marrow cell+1×107 spleen cell) of BALB/cH-2d mice was injected into CB6F1 mice through the tail vein,and these mice were randomly devided into the model group and the baicalin group. 6 SPF female mice were selected as the normal group. After modeling,the baicalin group was administered 30mg/(kg·d) of baicalin by gavage,and the model and normal groups were gavaged with normal saline of equal volume. Serum cytokines were detected after 14 days,and aGVHD scores and small intestinal mucosa pathdogy scores,immunohistochemical,and immunofluorescence detection were performed. Results A total of 2,131 different mRNAs were identified from patients with intestinal aGVHD and healthy volunteers. Kyoto Encyclopedia of Genes and Genomes analysis indicated that these mRNAs enriched the TNF signaling pathway and other pathways. The baicalin target was intersected with the above differential genes,and TNF-α and IL-2 were the primary baicalin targets in the treatment of intestinal aGVHD. The aGVHD scores and small intestinal mucosal pathology scores after baicalin treatment were significantly better than those in the model group,serum TNF-α and IL-2 levels were significantly decreased,and the TNF-α and IL-2 protein expression levels in intestinal immunohistochemistry and immunofluorescence were also significantly recovered (P<0.05). Conclusion Baicalin can reduce the inflammatory infiltration and destruction of intestinal mucosa by reducing TNF-α and IL-2 production and reducing the incidence of intestinal aGVHD after transplantation.关键词
黄芩苷/急性移植物抗宿主病/肿瘤坏死因子-α/白细胞介素-2/肠黏膜/网络药理学/小鼠Key words
baicalin/acute graft versus host disease/tumor necrosis factor-α/interleukin-2/intestinal mucosa/network pharmacology/mice分类
医药卫生引用本文复制引用
曹慧琴,韦玮,崔兴..黄芩苷调控炎性因子治疗肠道急性移植物抗宿主病的研究[J].北京中医药大学学报,2024,47(9):1272-1281,10.基金项目
国家自然科学基金面上项目(No.81774080) （No.81774080）
泰山学者青年专家人才项目(No.tsqn201812145)National Natural Science Foundation of China(No.81774080) （No.tsqn201812145）
