大学化学2024，Vol.39Issue(8)：212-220,9.DOI:10.3866/PKU.DXHX202312035
癸二酸-N-羟基琥珀酰亚胺酯的合成及应用
Synthesis and Application of Decanedioic Acid-N-Hydroxysuccinimide Ester:Exploration of Teaching Reform in Comprehensive Applied Chemistry Experiment
摘要
Abstract
This paper introduces a comprehensive applied chemistry experiment on the synthesis and application of an active ester cross-linking agent.The experiment involves the preparation of decanedioic acid-N-hydroxysuccinimide ester (NHS-DA),identification of its structure,and its application in improving collagen thermal stability.Initially,NHS-DA is synthesized from decanedioic acid,and N-hydroxysuccinimide using 1-(3-dimethylaminopropyl)-3-ethylcarbondiimide hydrochloride as a condensing agent,achieving a yield of approximately 85％.The product's structure is confirmed through nuclear magnetic resonance and Fourier-transform infrared spectroscopy,indicating the successful synthesis of high-purity NHS-DA.Subsequently,NHS-DA is applied to cross-link collagen sponge,with differential scanning calorimetry demonstrating enhanced thermal stability,thus promoting its application potential.The experiment is characterized by mild conditions,rapid reaction rates,high yields,and the recyclability of waste acetone,incorporating the use of various advanced instruments.It effectively integrates theoretical knowledge with practical application,fostering students'innovative thinking and expanding their scientific perspectives.关键词
癸二酸/N-羟基琥珀酰亚胺/胶原改性/应用化学综合实验Key words
Decanedioic acid/N-hydroxysuccinimide/Collagen modification/Comprehensive applied chemistry experiment分类
社会科学引用本文复制引用
沈里瑞,刘坤,杨颖,李冬婉,常文贵..癸二酸-N-羟基琥珀酰亚胺酯的合成及应用[J].大学化学,2024,39(8):212-220,9.基金项目
安徽省高校自然科学重点项目(2023AH052644) （2023AH052644）
皖西学院线上课程建设项目(wxxy2022045) （wxxy2022045）
皖西学院高层次人才启动资金项目(WGKQ2021040) （WGKQ2021040）
安徽省高等学校省级质量工程项目(2021zygzts067,2022zygzts123) （2021zygzts067,2022zygzts123）
