姜黄素、β-胡萝卜素对太湖猪精液常温保存效果的影响OA

In order to explore the effect of different types of phytogenic antioxidants on the preservation of Taihu pig semen at room temperature,the two commonly used antioxidants,curcumin and β-carotene,were selected.They were added at different concentrations(curcumin:0,3,6,9 µg·mL-1;β-carotene:0,10,20,40 µg·mL-1)to the semen diluent of Taihu pig.The sperm vitality,plasma membrane integrity rate and acrosome integrity rate were detected by using the visual observation,hypotonic swelling method and Wright-Giemsa staining method on the 1 st,3 rd,5 th and 7 th day of preservation at 17 °C.The results showed that the addition of certain concentrations of curcumin and β-carotene could significantly improve the sperm quality,with the optimal concentrations being 6µg·mL-1 for curcumin and 20µg·mL-1 for β-carotene.