口腔疾病防治2024，Vol.32Issue(10)：805-813,9.DOI:10.12016/j.issn.2096-1456.202440158
人诱导多能干细胞成骨分化的研究进展
Advances in the differentiation of human induced pluripotent stem cells into osteoblasts
摘要
Abstract
Bone diseases,such as osteoporosis and osteoarthritis,have emerged as pressing public health concerns requiring immediate attention and resolution.Cellular therapy and tissue engineering techniques are among the most promising therapeutic approaches for such conditions.Human induced pluripotent stem cells(hiPSCs)possess remark-able capacity for indefinite self-renewal in vitro and the ability to differentiate into all somatic cell types originating from the three germ layers,thereby making them a promising source of osteoblasts.Consequently,it is crucial to establish a well-delineated system for osteogenic differentiation of hiPSCs in vitro,with the aim to generate osteoblast-like cells that conform to clinical application standards.Numerous research teams have achieved substantial advancements in both the direct osteogenic differentiation of hiPSCs and the indirect pathway via mesenchymal stem cells.In this article,we pro-vide a comprehensive review of these two osteogenic differentiation pathways and their current applications,with the aim of serving as a valuable reference for bone regeneration technologies.Current research efforts have relied on embry-oid body formation and monolayer induction methods utilizing biomaterials to develop a system that facilitates in vitro culture and osteogenic differentiation of hiPSCs.However,the existing research is primarily constrained by unclear sys-tem components and low efficiency.Therefore,the development of a stepwise and three-dimensional induction system based on stringent regulation by specific compounds is a primary research direction for the future.关键词
骨再生/人诱导多能干细胞/成骨细胞/诱导分化/拟胚体法/单层诱导法/分阶段诱导法/三维定向/诱导体系Key words
bone regeneration/human induced pluripotent stem cells/osteoblast/induced differentiation/embryoid bodies method/monolayer culture method/defined stepwise differentiation method/three-dimensional orientation/induction system分类
医药卫生引用本文复制引用
廖凌子,宋雅朦,刘美萱,李思怡,周平..人诱导多能干细胞成骨分化的研究进展[J].口腔疾病防治,2024,32(10):805-813,9.基金项目
国家自然科学基金青年基金项目(81801855) This study was supported by the grants from National Natural Science Foundation Youth Foundation project(No.81801855). （81801855）
