基于CX3CL1/NF-κB信号通路探讨补肾强督方抑制M1巨噬细胞极化治疗强直性脊柱炎的机制研究OA北大核心CSTPCD
Mechanism of Bushen Qiangdu formula inhibiting M1 macrophage polarization by CX3CL1/NF-κB signaling pathway in the treatment of ankylosing spondylitis
目的:观察补肾强督方对CX3CL1/NF-κB信号通路介导的M1型巨噬细胞极化的影响,从而阐明其治疗强直性脊柱炎的分子机制.方法:采用脂多糖(LPS)诱导THP-1细胞构建细胞模型,并给予补肾强督方含药血清和CX3CL1 抑制剂AZD8797 进行干预.采用酶联免疫吸附试验检测细胞炎症因子(TNF-α、IL-6、IL-1β和IL-17)的含量;采用流式细胞术检测CD86的表达;采用蛋白印迹检测iNOS、破骨分化标记蛋白(TRAP、Calciton…查看全部>>
Objective:To observe the effect of Bushen Qiangdu formula on CX3CL1/NF-κB signaling pathway mediated po-larization of M1 macrophages and elucidate its molecular mechanism in treating ankylosing spondylitis.Methods:Lipopolysaccha-ride(LPS)-induced THP-1 cells were used to construct a cell model,and Bushen Qiangdu medicated serum and CX3CL1 inhibi-tor AZD8797 were given for intervention.The levels of inflammatory factors(TNF-α,IL-6,IL-1β and IL-17)were detecte…查看全部>>
吴琳;李松倍;苏晓庆;韩天然;李泽光
黑龙江中医药大学,黑龙江 哈尔滨 150040||黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040黑龙江中医药大学,黑龙江 哈尔滨 150040黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040黑龙江中医药大学,黑龙江 哈尔滨 150040黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040
中医学
补肾强督方CX3CL1/NF-κB信号通路巨噬细胞极化破骨细胞分子机制
Bushen Qiangdu formulaCX3CL1/NF-κB signaling pathwayMacrophage polarizationOsteoclastsMolecu-lar mechanism
《海南医学院学报》 2024 (19)
1478-1485,8
This study was supported by Norman Bethune Medical Science Research Fund(TY144EN) 白求恩医学科学研究基金(TY144EN)
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