海南师范大学学报（自然科学版）2024，Vol.37Issue(3)：307-312,6.DOI:10.12051/j.issn.1674-4942.2024.03.006
基于石墨炔-金复合材料修饰电极的电化学基因传感器对大肠杆菌特征序列的检测
Detection of Specific Sequences of Escherichia coli by Electrochemical Gene Sensors Based on Graphyne-Au Composite Modified Electrode
摘要
Abstract
In this paper,we prepared a graphyne-Au nanocomposite modified carbon ionicliquid electrode(Au/GDY/CILE)as the working electrode using drop-coating and constant potential deposition methods.Cyclic voltammetry(CV)experiments showed that the Au-GDY composite could effectively improve the electrochemical properties of the modified electrode interface,and the modified probe ssDNA was successfully immobilized onto the surface of the working electrode based on gold-sulfur bond self-as-sembly effect,which was used to construct a gene sensor for the detection of specific sequences of Escherichia coli(E.coli).By us-ing methylene blue(MB)as an electrochemical indicator,the sensor demonstrated a broad detection range(1.0×10-15 mol/L to 1.0×10-7 mol/L)and an impressively low detection limit(3.17×10-16 mol/L)and high sensitivity[2.91 µA/(M·cm2)]for the detection of characteristic gene sequence of E.coli through the application of differential pulse voltammetry(DPV).关键词
石墨炔-金/纳米复合材料/电化学基因传感器/大肠杆菌特征序列/亚甲基蓝Key words
Au/GDY/nanocomposite/electrochemical gene sensors/characteristic gene sequence of E.coli/methylene blue分类
化学化工引用本文复制引用
王丽思,黄郅郯,周娟,刘涛,闫丽君,孙伟..基于石墨炔-金复合材料修饰电极的电化学基因传感器对大肠杆菌特征序列的检测[J].海南师范大学学报（自然科学版）,2024,37(3):307-312,6.基金项目
海南省自然科学基金项目(824QN266) （824QN266）
国家自然科学基金项目(21964007) （21964007）
