海南师范大学学报（自然科学版）2024，Vol.37Issue(3)：313-319,7.DOI:10.12051/j.issn.1674-4942.2024.03.007
光谱法研究Pb2+和Cd2+与海南木榄叶DNA的相互作用
Study on the Interaction between Pb2+,Cd2+and DNA of Hainan Bruguiera gymnorhiza Leaf by Spectroscopic Method
摘要
Abstract
Bruguiear gymnorrhiza is one kind of medicinal mangrove plants with higher commercial value,which also has good protective effect on the ecology of the coastal area.In this study,the interaction of two kinds of heavy metals including Pb2+,Cd2+with the DNA extracted from the leafs of Hainan Bruguiear gymnorrhiza were investigated by using UV-vis and fluorescence spectroscopes.The results indicated that the presence of Pb2+,Cd2+had a certain influence on the structure of DNA and the interaction could occur in a groove-binding mode.The thermodynamic parameters showed that it is a sponta-neous reaction between Pb2+and DNA with hydrogen bond and Vander Waals force as the main interaction forces.Howev-er,Cd2+unspontaneously interacted with DNA and hydrophobic force played a main role in the binding process.This re-search can provide some theoretical support for further understanding the mechanism of mangrove plants' tolerance of heavy metal ions.关键词
木榄叶/DNA/重金属离子/光谱法/相互作用Key words
Bruguiera gymnorhiza leaf/DNA/heavy metal ions/spectroscopic method/the interaction分类
医药卫生引用本文复制引用
陈婉慧,宋媛,陈光英,何文英..光谱法研究Pb2+和Cd2+与海南木榄叶DNA的相互作用[J].海南师范大学学报（自然科学版）,2024,37(3):313-319,7.基金项目
国家自然科学基金项目(22177023) （22177023）
海南省科技计划重点研发项目(ZDYF2022XDNY197) （ZDYF2022XDNY197）
