海南师范大学学报（自然科学版）2024，Vol.37Issue(3)：357-361,370,6.DOI:10.12051/j.issn.1674-4942.2024.03.013
致聋鸟模型的制作及其在聋哑机制研究中的应用
Preparation and Application of Deafened Avian Model in the Study of Deaf-mutism Mechanism
摘要
Abstract
The creation of deaf-mute birds through the induction of deafness in juvenile birds and the establishment of adult deafened bird models present an optimal experimental approach for investigating the mechanisms underlying deaf-mutism formation subsequent to juvenile deafness.Utilizing the Blood/Cell/Tissue Genomic DNA Extraction Kit(DP304-02)for genomic DNA extraction,the gender of juvenile birds was determined based on the number of electro-phoretic bands formed at 200 bp.Employing fine hooks to grasp the base of the cochlea,the cochlea of the juvenile bird was extracted,and the integrity of the extracted cochlea was examined under a microscope.Juvenile birds subject-ed to deafening will gradually transition into deaf-mute birds.Sonogram software was utilized to analyze the spectro-gram and phonetic parameters of their bird songs.Analyzing the gradual distortion process of the spectrogram of juve-nile deafened birds and the singing patterns of adult deafened birds was conducive to investigating the mechanism of deaf-mutism formation.Studying the degeneration of the auditory and vocal central nervous system of deafened birds using electron microscopy technology can offer valuable insights for fundamental research in this field.关键词
致聋鸟方法/性别鉴定/聋哑测试/鸟脑电镜切片/白腰文鸟Key words
deafened bird methodologies/gender determination/deaf-mute assessment/avian brain electron microscope sections/Lonchura striata分类
生物科学引用本文复制引用
张信文,杨思远,林春,曾少举..致聋鸟模型的制作及其在聋哑机制研究中的应用[J].海南师范大学学报（自然科学版）,2024,37(3):357-361,370,6.基金项目
海南省自然科学基金项目(319QN238) （319QN238）
国家自然科学基金项目(31860269) （31860269）
