超临界CO2流体技术制备脱细胞外基质材料及其生物医学应用OACSTPCD

Decellularized extracellular matrix(dECM)materials play a significant role in tissue engineering and regenerative medicine.Supercritical CO2 fluid(SCF-CO2)technology has been proven to be applicable in the preparation of dECM materials.By reviewing relevant literature in recent years,this paper summarizes the basic principles of SCF-CO2,the mechanisms of decellularization and sterilization by SCF-CO2,and discusses the impact of auxiliary solvents and pretreatment techniques on SCF-CO2-assisted decellularization,as well as the biomedical applications of dECM materials.The results show that,compared with conventional methods,SCF-CO2 with high permeability and high diffusivity could maintain the structural integrity and biological activ-ity of extracellular matrix(ECM)more effectively and show the advantage of high sterilization efficiency.Mo-reover,the prepared dECM materials have significant potential for application in the field of tissue repair and regenerative medicine.