多拷贝pucBA基因在沼泽红假单胞菌光合生长中的作用OACSTPCD

Selecting Rhodopseudomonas palustris CGA009 strain,gene knockout method was used to sequen-tially knock out five pairs of pucBA genes in the strain in order to explore the effects of different pucBA genes on photosynthetic growth,spectral phenotype and photosynthetic pigment mass concentration of the strain.The results showed that the expression levels of different pucBA genes were positively correlated with the pho-tosynthetic growth rate and photosynthetic pigment synthesis of the strain.The pucBAb and pucBAa genes played a major role in strain growth under high light conditions,while the pucBAb,pucBAA and pucBAa genes played a major role in strain growth under low light conditions.Knocking out the pucBAd gene could lead to efficient expression of pucBAb gene,while knocking out the pucBAc gene could increase LH1-RC synthesis,indicating that the knockout of a certain pucBA gene had a compensatory effect on the expression of other pho-tosynthetic genes.