华侨大学学报（自然科学版）2024，Vol.45Issue(5)：618-625,8.DOI:10.11830/ISSN.1000-5013.202311010
沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用
Regulatory Role of Arsenic Regulatory Protein(ArsR)in Rhodopseudomonas palustris CGA009
摘要
Abstract
Using a modified gel electrophoretic mobility shift assay(EMSA)method and Arsenic biosensing method,the regulation of six highly heterogeneous ArsR-associated proteins from Rhodopseudomonas palustris CGA009 bacterial strain is investigated.The reseach results show that six ArsR-associated proteins exhibited As(Ⅲ)-dependent regulatory activity.The modified EMSA mappings can systematically evaluate the functions of ArsR.Six ArsR-associated proteins have different selectivity in regulating the arsenic resistance o-peron of different species(E.coli pR733 or Staphylococcus aureus p Ⅰ 258).ArsRs with amino acid sequence homology(even with below 30％ homology)have arsenic-dependent regulation.关键词
沼泽红假单胞菌/砷/砷调控蛋白/砷生物传感Key words
Rhodopseudomonas palustris/arsenic/arsenic regulatory protein/arsenic biosensing分类
生物科学引用本文复制引用
金春英,崔亮,陈勇男,杨素萍,赵春贵..沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用[J].华侨大学学报（自然科学版）,2024,45(5):618-625,8.基金项目
国家海洋公益性行业科研专项(201505026) （201505026）
福建省自然科学基金资助项目(2018J01049) （2018J01049）
