沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用OACSTPCD
Regulatory Role of Arsenic Regulatory Protein(ArsR)in Rhodopseudomonas palustris CGA009
采用改良的凝胶电泳迁移滞缓(EMSA)法和砷生物传感法研究沼泽红假单胞菌CGA009菌株6个高度异质性ArsR相关蛋白的调控作用.研究结果表明:6个ArsR相关蛋白都具有As(Ⅲ)依赖的调控活性,改良的EMSA图谱可用于系统评价ArsR的功能;6个相关蛋白对异种(E.coli pR733 or Stapylococcus aureus pⅠ 258)的砷抗性操纵子的调控具有不同的选择性,氨基酸序列同源性(甚至低于30％同源性)的ArsR具有砷依赖的调控作用.
Using a modified gel electrophoretic mobility shift assay(EMSA)method and Arsenic biosensing method,the regulation of six highly heterogeneous ArsR-associated proteins from Rhodopseudomonas palustris CGA009 bacterial strain is investigated.The reseach results show that six ArsR-associated proteins exhibited As(Ⅲ)-dependent regulatory activity.The modified EMSA mappings can systematically evaluate the functions of ArsR.Six ArsR-associated proteins have different selectivity in regulating the arsenic resistance o-peron of different species(E.coli pR733 or Staphylococcus aureus p Ⅰ 258).ArsRs with amino acid sequence homology(even with below 30％ homology)have arsenic-dependent regulation.
金春英;崔亮;陈勇男;杨素萍;赵春贵
华侨大学化工学院,福建厦门 361021北京农学院国际学院,北京 102206
生物工程
沼泽红假单胞菌砷砷调控蛋白砷生物传感
Rhodopseudomonas palustrisarsenicarsenic regulatory proteinarsenic biosensing
《华侨大学学报（自然科学版）》 2024 (005)
618-625 / 8
国家海洋公益性行业科研专项(201505026);福建省自然科学基金资助项目(2018J01049)
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