华侨大学学报（自然科学版）2024，Vol.45Issue(5)：626-634,9.DOI:10.11830/ISSN.1000-5013.202311032
产灵菌红素沙雷氏菌R18的鉴定及基因组特性
Identification and Genomic Characterization of Prodigiosin-Producing Serratia sp.R18
摘要
Abstract
Strain R18 was identified using morphological,physiological,biochemical and molecular biological methods.Its taxonomic status and biological properties were further analyzed by comparing genome sequencing and bioinformatics,and the synthetic gene cluster and metabolic pathway of the red pigment in this strain were explored at the genomic level.The results showed that the optimal growth conditions for strain R18 were tem-perature of 30-37 ℃,pH value of 6-8,and the mass concentration of NaCl of 0-10 g·L-1.Strain R18 shared a 16S rDNA similarity of 99.86％ to Serratia marcescens type strain ATCC 13880,the genome average nucle-otide identity value and the digital DNA-DNA hybridization value were 98.73％ and 89.5％,respectively,both of which were higher than the species identification threshold and belong to the same specie.Strain R18 prodigiosin synthetic gene cluster had a total length of 35 021 bp and contained 29 genes including 4 core syn-thetic genes,10 complementary synthetic genes,1 regulatory gene,1 transporter gene and 13 other genes,it had a complete prodigiosin synthesis metabolic pathway.关键词
粘质沙雷氏菌/灵菌红素/菌种鉴定/基因组特性Key words
Serratia marcescens/prodigiosin/bacterial identification/genomic characterization分类
生物科学引用本文复制引用
陈明霞,邢元军,李和阳,施俊豪..产灵菌红素沙雷氏菌R18的鉴定及基因组特性[J].华侨大学学报（自然科学版）,2024,45(5):626-634,9.基金项目
福建省海洋经济发展专项资金资助项目(FJHJF-L-2022-11) （FJHJF-L-2022-11）
华侨大学2023年实验教学与管理改革课题(SY2023J05) （SY2023J05）
福建省海洋生态保护与修复重点实验室开放基金资助项目(EPR2020002) （EPR2020002）
