解放军医学杂志2024，Vol.49Issue(9)：1038-1044,7.DOI:10.11855/j.issn.0577-7402.0025.2024.0605
烟草烟雾与机动车尾气暴露建立的大鼠慢性阻塞性肺疾病模型比较
A comparative study of chronic obstructive pulmonary disease models in rats established by exposure to cigarette smoke and motor vehicle exhaust
摘要
Abstract
Objective To compare the fidelity of chronic obstructive pulmonary disease(COPD)models established using two methods:exposure to cigarette smoke(CS)and exposure to motor vehicle exhaust(MVE)in rats.Methods Twenty-four male SD rats were randomly divided into control,CS-exposed(CS),and MVE-exposed(MVE)groups,with 8 rats per group.Rats in CS and MVE groups were exposed to CS or MVE,respectively,to induce COPD models.After COPD model established,lung function of each group was assessed.Bronchoalveolar lavage fluid(BALF)was collected to measure inflammatory cell counts,levels of inflammatory cytokines interleukin-6(IL-6)and tumor necrosis factor(TNF)-α,and expression levels of mucin 5AC(MUC5AC).Lung tissue sections were stained with hematoxylin and eosin(HE)to observe pulmonary tissue and airway pathological changes.Periodic acid-Schiff(PAS)staining was used to detect goblet cell hyperplasia in airways.Results Compared with control group,rats in CS and MVE groups showed significantly increased inspiratory resistance(RI),total lung capacity(TLC),and lung static compliance(Cchord)(P<0.05),while expiratory flow parameters FEV50/FVC were significantly decreased(P<0.05).Compared with MVE group,rats in CS group had significantly higher RI,TLC,and Cchord(P<0.05),and lower FEV50/FVC(P<0.05).HE staining of lung tissues showed that mean linear intercept(MLI)was significantly higher in both CS and MVE groups compared with control group(P<0.05),with CS group having higher MLI than MVE group(P<0.05).BALF analysis revealed that white blood cells,neutrophils,macrophages,lymphocytes,IL-6,and TNF-α levels were significantly higher in both CS and MVE groups compared with control group(P<0.05),and inflammatory cell counts,IL-6,and TNF-α levels were higher in CS group compared with MVE group(P<0.05).PAS staining of lung tissues indicated that goblet cells in large airways were significantly increased in both CS and MVE groups compared with control group(P<0.05),with CS group showing higher goblet cell counts than MVE group(P<0.05).Expression levels of MUC5AC in BALF were significantly higher in both CS and MVE groups compared with control group(P<0.05),with CS group having significantly higher MUC5AC levels than MVE group(P<0.05).Conclusions Exposure to CS or MVE can establish a rat model of COPD,with CS exposure better mimicking characteristics of acute exacerbation of COPD compared to MVE exposure.关键词
慢性阻塞性肺疾病/烟草烟雾/机动车尾气/动物模型Key words
chronic obstructive pulmonary disease/cigarette smoke/motor vehicle exhaust/animal model分类
临床医学引用本文复制引用
李德富,叶园园,章洪萍,侯润华,何耀军,张春云,胡杰英,陈蕊..烟草烟雾与机动车尾气暴露建立的大鼠慢性阻塞性肺疾病模型比较[J].解放军医学杂志,2024,49(9):1038-1044,7.基金项目
This work was supported by the National Natural Science Foundation of China(81900033),the Natural Science Foundation of Guangdong Province(2022A1515012504),the Basic Research Project of Guangzhou(202201020513),and the Guangzhou Key Medical Discipline(2021-2023 Project) 国家自然科学基金(81900033) （81900033）
广东省自然科学基金(2022A1515012504) （2022A1515012504）
广州市基础研究计划项目(202201020513) （202201020513）
广州市医学重点学科(2021-2023项目) （2021-2023项目）
