心俞穴位埋线联合曲美他嗪对大鼠心肌梗死后能量代谢影响的研究OA北大核心CSTPCD

Objective:To observe the impact of acupoint catgut embedding at Xinshu combined with trimetazidine on energy metabolism after myocardial infarction(MI)in rats.Methods:The MI rat model was constructed by the typical ligation of the left anterior descending branch of the coronary artery,and the successfully-modeled rats were randomly divided into the model group,the catgut embedding group,the western medicine group,and the combined group,another single thoracotomy without ligation was used as the sham operation group.The intervention was started on the second day after operation,and the observation was car-ried out at two different time points of 7 d and 28 d after operation.The pathological morphology of myocardial tissue was observed by hematoxylin-eosin(H&E)staining,the content of adenosine triphosphate(ATP)in myocardial tissue and the concentration of free fatty acid(FFA)in serum were determined by biochemical detection,and the expression of AMPK and GLUT4 in myocardi-al tissue was detected by western blot.Results:In model group at 7 d and 28 d after model construction,the myocardial cells were swollen and disorganized,and obvious punctate inflammatory cell infiltration were seen compared with the sham peration group.Cardiomyocyte cross-sectional area and serum FFA concentration were increased,and myocardial ATP content and expression of AMPK and GLUT4 were decreased(all P<0.05).At 7 d and 28 d after operation,compared with the model group,the morphol-ogy and arrangement of myocardial cells and the degree of inflammatory cell infiltration in the three treatment groups were im-proved to varying degrees,the cross-sectional area of myocardial cells and the concentration of serum FFA were decreased to vary-ing degrees,and the content of myocardial ATP and the expression of AMPK and GLUT4 protein were increased to varying de-grees(all P<0.05),and the curative effect of the combined group was more obvious than that of the catgut embedding group and the western medicine group(P<0.05).Compared with the treatment groups at 7 d after operation,the cross-sectional area of myocardial cells,myocardial ATP content and myocardial AMPK and GLUT4 protein expression in each treatment group in-creased at 28 d after operation(all P<0.05),and the improvement effect of the combined group was the most obvious(P<0.05).Conclusion:Acupoint catgut embedding at Xinshu combined with trimetazidine can further exert the protective effect on the myo-cardium after MI,and the combined application of the two has a synergistic effect.The mechanism may be to increase the expres-sion of AMPK and GLUT4 protein in myocardial tissue,reduce serum FFA concentration and increase myocardial ATP content to improve myocardial energy metabolism disorder after MI,thereby improving cardiomyocyte hypertrophy and promoting myocar-dial injury repair.