河北医学2024，Vol.30Issue(9)：1428-1434,7.DOI:10.3969/j.issn.1006-6233.2024.09.03
芍药苷调节SIRT1/PGC-1α/Nrf2信号通路对过氧化氢诱导皮肤成纤维细胞氧化应激损伤的影响
Effect of Paeoniflorin on Hydrogen Peroxide-Induced Oxidative Stress Damage in Skin Fibroblasts by Regulating the SIRT1/PGC-1α/Nrf2 Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of paeoniflorin(PF)on oxidative stress damage induced by hydrogen peroxide(H2O2)in skin fibroblasts(HSF)by regulating the silent information regulator 2 homo-logue 1(SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α(PGC-1α)/nuclear fac-tor erythroid-derived 2-related factor 2(Nrf2)signaling pathway.Methods:HSF cells were studied and sep-arated into the Control group,H2O2 group,L-PF,M-PF,H-PF group,and PF+EX527 group.CCK-8 was applied to detect HSF cell proliferation.The β-galactosidase staining method was applied to detect HSF cell aging.Flow cytometry was used to detect apoptosis of HSF cells.ELISA method was used to detect the expres-sion of ROS,SOD,GSH,and MDA in HSF cells.WB was used to detect the expressions of SIRT1,PGC-1α,and Nrf2 proteins in HSF cells.Results:The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 ex-pression of HSF cells in the H2O2 group were lower than in the control group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the control group(P<0.05).Compared with the H2O2 group,the survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression were increased in the L-PF group,M-PF group,and H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were decreased(P<0.05).The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression in the PF+EX527 group were lower than in the H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the H-PF group(P<0.05).Conclusion:PF can inhibit H2O2-induced oxidative stress damage in HSF cells,and its mechanism may be achieved by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.关键词
芍药苷/沉默信息调节因子2同系物1/氧化物酶体增殖物激活受体γ共激活剂-1α/核因子E2相关因子2/皮肤成纤维细胞/氧化应激损伤Key words
Paeoniflorin/Silent information regulator 2 homologue 1/Peroxisome proliferator ac-tivated receptor-gamma coactivator-1α/Nuclear factor erythroid-derived 2-related factor 2/Skin fibro-blasts/Oxidative stress damage引用本文复制引用
黄燕,杨艳清,周进飞..芍药苷调节SIRT1/PGC-1α/Nrf2信号通路对过氧化氢诱导皮肤成纤维细胞氧化应激损伤的影响[J].河北医学,2024,30(9):1428-1434,7.基金项目
湖北省武汉市医学科研项目立项任务书,(编号:WX20Q03) （编号:WX20Q03）
