实用临床医药杂志2024，Vol.28Issue(17)：9-14,6.DOI:10.7619/jcmp.20240996
FOXO1、SMAD4在食管癌组织中的表达水平及其与临床病理特征和预后的关系
Expression levels of FOXO1 and SMAD4 in esophageal cancer tissues and their relationships with clinicopathological features and prognosis
摘要
Abstract
Objective To investigate the expression levels of FOXO1 and SMAD4 in esophageal canc-er(EC)tissues and their associations with clinicopathological features and prognosis.Methods Tissue samples of cancerous and adjacent non-cancerous tissues were collected from 131 EC patients.Hema-toxylin-eosin(HE)staining was performed to observe the pathological morphology of EC and adjacent tissues.The mRNA expression of FOXO1 and SMAD4 was measured using quantitative real-time poly-merase chain reaction(qRT-PCR),while the protein expression of FOXO1 and SMAD4 was deter-mined by immunohistochemistry;Spearman's correlation analysis was employed to assess the correla-tion between FOXO1 and SMAD4 protein expression in EC tissues;Cox regression analysis was con-ducted to analyze factors influencing the prognosis of EC patients;Kaplan-Meier analysis was used to perform survival analysis for EC patients.Results The expression of FOXO1 mRNA and its protein positive expression rate were significantly higher in the EC tissues than those in the adjacent tissues,whereas the expression of SMAD4 mRNA and its protein positive expression rate were significantly lower(P＜0.05).FOXO1 and SMAD4 were associated with tumor diameter,tumor differentiation,lymph node metastasis and TNM stage(P＜0.05).A negative correlation was observed between FOXO1 and SMAD4 protein expression in EC tissues(r=-0.419,P＜0.05).FOXO1,SMAD4,tumor diameter,differentiation,lymph node metastasis and TNM stage were identified as factors in-fluencing mortality in EC patients(P＜0.05).The 3-year overall survival rate of EC patients was 84.73％(111/131).The 3-year cumulative survival rate was significantly lower in the EC patients with positive FOXO1 expression than in thosewith negative FOXO1 expression(P＜0.05),while it was significantly higher in patients with positive SMAD4 expression than in those with negative SMAD4 expression(P＜0.05).Conclusion The positive expression rate of FOXO1 is higher,while that of SMAD4 is lower in EC tissues.Their protein expressions were associated with clinico-pathological features and prognosis in EC patients.FOXO1 and SMAD4 may serve as important bio-markers for predicting the prognosis of EC.关键词
食管癌/FOXO1蛋白/SMAD4蛋白/临床病理特征/预后Key words
esophageal cancer/FOXO1 protein/SMAD4 protein/clinicopathological fea-tures/prognosis分类
医药卫生引用本文复制引用
关守涛,王登正,胡锋超,宁琳琳,安月,耿惠,王玉强..FOXO1、SMAD4在食管癌组织中的表达水平及其与临床病理特征和预后的关系[J].实用临床医药杂志,2024,28(17):9-14,6.基金项目
河北省邢台市重点研发计划项目(2022ZC081) （2022ZC081）
