河北医学2024，Vol.30Issue(9)：1473-1478,6.DOI:10.3969/j.issn.1006-6233.2024.09.011
茶黄素调节PI3K/Akt/mTOR信号通路对A549细胞增殖凋亡及自噬的影响
Effects of Theaflavin on Proliferation Apoptosis and Autophagy of A549 Cell by Regulating the PI3K/Akt/mTOR Signaling Pathway
摘要
Abstract
Objective:To investigate the effects of theaflavin on A549 cell proliferation,apoptosis,and autophagy by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian tar-get of rapamycin(mTOR)signaling pathway.Methods:The proliferation rate of A549 cells was measured af-ter intervention with 0,2.5,5,10,20,40,80,and 160 μmol/L theaflavin,and the appropriate concentra-tion of action was screened.A549 cells were separated into control group,low theaflavin group(20 μmol/L theaflavin),medium theaflavin group(40 μmol/L theaflavin),high theaflavin group(80 μmol/L theafla-vin),and high theaflavin+740Y-P group(80 μmol/L theaflavin+30 μmol/L PI3K/Akt/mTOR signaling pathway activator 740Y-P).CCK-8 method and plate colony formation experiment were applied to determine the proliferation of A549 cells.Flow cytometry and MDC methods were applied to determine apoptosis and au-tophagic vacuole formation in A549 cells,respectively.Western blot was applied to determine the expression of PI3K/Akt/mTOR signaling pathway proteins and autophagy proteins in A549 cells.Results:Compared with 0 μmol/L of theaflavin,2.5,5,10,20,40,80,and 160 μmol/L of theaflavin can inhibit the prolifera-tion of A549 cells.The IC50 value of theaflavin on A549 cells was calculated to be 36.72 μmol/L.Compared with the control group,the proliferation rate,colony formation rate,p-PI3K/PI3K,p-Akt/Akt,and p-mTOR/mTOR of A549 cells in the low theaflavin group,medium theaflavin group,and high theaflavin group all reduced,the apoptosis rate,relative content of autophagic vacuoles,LC3II/LC3I,and Beclin-1 protein expression all increased,and all showed dose-dependent changes in theaflavin(P<0.05).Compared with the high theaflavin group,the proliferation rate,colony formation rate,p-PI3K/PI3K,p-Akt/Akt,and p-mTOR/mTOR of A549 cells in the high theaflavin+740Y-P group all increased,the apoptosis rate,relative content of autophagic vacuoles,LC3II/LC3I,and Beclin-1 protein expression all reduced(P<0.05).Con-clusion:Theaflavin can induce protective autophagy by inhibiting the activation of the PI3K/Akt/mTOR sig-naling pathway,thereby promoting apoptosis and inhibiting the proliferation of lung cancer cells.关键词
茶黄素/磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路/自噬/肺癌Key words
Theaflavin/Phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapa-mycin signaling pathway/Autophagy/Lung cancer引用本文复制引用
段兴隆,张华..茶黄素调节PI3K/Akt/mTOR信号通路对A549细胞增殖凋亡及自噬的影响[J].河北医学,2024,30(9):1473-1478,6.基金项目
陕西省汉中市人民医院项目计划,(编号:sy-2023-02) （编号:sy-2023-02）
