河北医学2024，Vol.30Issue(9)：1514-1519,6.DOI:10.3969/j.issn.1006-6233.2024.09.019
血清miR-22 miR-98-5p表达与非小细胞肺癌三维适形放疗疗效的关系研究
Relationship between Expressions of Serum miR-22 and miR-98-5p and Efficacy of Three-Dimensional Conformal Radiotherapy for Non-Small Cell Lung Cancer
摘要
Abstract
Objective:To observe the differences in the expression levels of microRNA(miR)-22 and miR-98-5p in patients with non-small cell lung cancer(NSCLC)and different efficacies after three-dimen-sional conformal radiotherapy,and to analyze the association between expression levels and efficacy.Meth-ods:A total of 104 patients with NSCLC who were admitted to the hospital from January 2020 to January 2024 were enrolled.All patients received three-dimensional conformal radiotherapy.After radiotherapy completion,the expression levels of miR-22 and miR-98-5p were determined by real-time fluorescence quantitative PCR.One-way analysis of variance was used to analyze the differences in the expression levels of miR-22 and miR-98-5p in patients with different efficacies.Spearman correlation analysis was applied to investigate the correlation with radiotherapy efficacy.The logistic multivariate regression model was adopted to analyze the related factors affecting the efficacy of radiotherapy.The receiver operating characteristic(ROC)curve was drawn to explore the evaluated efficiency of miR-22 and miR-98-5p expression levels on the effectiveness of radiotherapy.Results:There were statistically significant differences in the expression levels of miR-22 and miR-98-5p among patients with different efficacies(P<0.05).There was a statistical significance in the ex-pression level of miR-22 between the SD group and the PD group(P<0.05).The expression levels of miR-22 and miR-98-5p revealed statistical differences in the CR group and PR group compared with those in the SD group and PD group(P<0.05).There were statistical differences in the expression levels of miR-22 and miR-98-5p between CR group and PR group(P<0.05).Univariate analysis showed that different pathologi-cal types,TNM stages,differentiation degrees,and expression levels of miR-98-5p and miR-22 were related to the efficacy of radiotherapy(P<0.05).Logistic regression model analysis revealed that TNM staging(OR=3.360,95%CI:1.229-9.184),differentiation degree(OR=2.779,95%CI:1.066-7.246),miR-98-5p expression level(OR=2.085,95%CI:1.147-3.792),and miR-22 expression level(OR=0.590,95%CI:0.368-0.946)were the influencing factors for evaluating the efficacy of radiotherapy(P<0.05).ROC curve suggested that the areas under the curves(AUCs)of miR-98-5p and miR-22 in evaluating the effectiveness of radiotherapy were 0.798 and 0.800,respectively,the sensitivities were 0.589 and 0.630,and the speci-ficities were 0.871 and 0.839 respectively.The AUC of miR-98-5p+miR-22 was 0.895,significantly high-er than that of a single indicator(P<0.05),and the sensitivity and specificity were 0.726 and 0.935.Con-clusion:There are some differences in the expression levels of serum miR-98-5p and miR-22 among patients with different efficacies after three-dimensional conformal radiotherapy for NSCLC,and the expression levels are significantly related to the effectiveness of radiotherapy and have certain evaluated value on efficacy.关键词
非小细胞肺癌/三维适形放疗/miR-22/miR-98-5p/相关性Key words
Non-small cell lung cancer/Three-dimensional conformal radiotherapy/miR-22/miR-98-5p/Correlation引用本文复制引用
汤宇,赵玉魁,孙祝..血清miR-22 miR-98-5p表达与非小细胞肺癌三维适形放疗疗效的关系研究[J].河北医学,2024,30(9):1514-1519,6.基金项目
2022年安徽省重点研究与开发计划项目,(编号:2022e07020070) （编号:2022e07020070）
