军事医学2024，Vol.48Issue(9)：656-663,8.DOI:10.7644/j.issn.1674-9960.2024.09.003
低氧与过氧化氢预处理调控间充质干细胞归巢能力的比较研究
A comparative study of regulation of the homing ability of mesenchymal stem cells by hypoxia and hydrogen peroxide pretreatment
摘要
Abstract
Objective To determine the optimal conditions for CXCR4 upregulation by comparing the expression levels of chemokine(C-X-C motif)receptor 4(CXCR4)in MSCs cultured with varying concentrations of oxygen and hydrogen peroxide(H2O2).Methods MSCs were cultured with 0.1％,1％,or 3％O2 and 50 μmol/L H2O2 for different lengths of time(3,6,12,and 24 h).The mRNA and protein expressions of CXCR4 in MSCs were measured by real-time quantitative PCR(qPCR),Western blotting,and immunofluorescence staining.The viability and chemotactic ability of MSCs were measured using CCK-8,wound-healing and Transwell migration assays.Results Both hypoxia and H2O2 treatment were found to upregulate MSC expressions of CXCR4 to some extent.The mRNA and protein levels of CXCR4 were higher after 6-12 h of culture of MSCs with 3％O2,and significantly higher when treated with H2O2 for 6 h.Cell viability was significantly increased after culture with 3％O2 compared with the control group and both 3％O2 and H2O2 pretreatment could enhance chemotactic migration in MSCs.Conclusion Culture with 3％O2 and H2O2 pretreatment can upregulate CXCR4 expressions in MSCs and enhance migration in cells,with superior effects observed with 3％O2.Therefore,treatment with 3％O2 represents the best choice for upregulating the chemotactic ability of MSCs.关键词
间充质干细胞/战创伤/趋化因子(C-X-C基序)受体4/归巢/低氧/H2O2Key words
mesenchymal stem cells/war wound/CXCR4/homing/hypoxia/H2O2分类
医药卫生引用本文复制引用
黄一琛,姜明霞,马文静,陈巧汨,白睿,修冰水,宋梦文,牛洁,刘志强..低氧与过氧化氢预处理调控间充质干细胞归巢能力的比较研究[J].军事医学,2024,48(9):656-663,8.基金项目
国家自然科学基金项目(82200295,81971168) （82200295,81971168）
