青岛大学学报（医学版）2024，Vol.60Issue(4)：487-490,4.DOI:10.11712/jms.2096-5532.2024.60.046
D-AP5对PD小鼠黑质多巴胺能神经元簇状放电影响
Effect of intracerebroventricular injection of D-AP5 on burst firing of dopaminergic neurons in the substantia nigra in a mouse model of Parkinson's disease
摘要
Abstract
Objective To investigate the effect of intracerebroventricular injection of D(-)-2-amino-5-phosphonopentanoic acid(D-AP5)on the burst firing of dopaminergic neurons in the substantia nigra ina mouse model of Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Mice were randomly divided into control group,D-AP5 group,MPTP group,and D-AP5+MPTP group.The bursting rate of substantia nigra dopaminergic neurons was recorded through the electrophysiological test.Results The factorial analysis of variance indicated an interaction between D-AP5 and MPTP(FMPTP=4.601,P＜0.05;FD-AP5=2.399,P＞0.05;FMPTP×D-AP5=12.020,P＜0.01).The individual effect analysis showed that there was no significant difference in the bursting rate of substantia nigradopaminergic neurons between the D-AP5 group and the control mice(F=1.916,P＞0.05);and the D-AP5+MPTP group showed a significantly lower bursting rate compared with the MPTP group(F=12.094,P＜0.01).Conclusion Intracerebroventricular injection of D-AP5 can reduce the bursting rate of do-paminergic neurons in the substantia nigra of MPTP model mice.关键词
帕金森病/受体,N-甲基-D-天冬氨酸/兴奋性氨基酸拮抗剂/黑质/多巴胺能神经元/电生理学/小鼠,近交C57BLKey words
Parkinson disease/receptors,N-methyl-D-aspartate/excitatory amino acid antagonists/substantia nigra/do-paminergic neurons/electrophysiology/mice,inbred C57BL分类
医药卫生引用本文复制引用
赵继虎,刘恒,孙鹏..D-AP5对PD小鼠黑质多巴胺能神经元簇状放电影响[J].青岛大学学报（医学版）,2024,60(4):487-490,4.基金项目
国家自然科学基金青年科学基金项目(32000795) （32000795）
中国博士后基金面上项目(2017M622129) （2017M622129）
山东省自然科学基金青年科学基金项目(ZR2022MH022,ZR2020QC095) （ZR2022MH022,ZR2020QC095）
