青岛大学学报（医学版）2024，Vol.60Issue(4)：491-495,5.DOI:10.11712/jms.2096-5532.2024.60.092
Exendin-4对小鼠黑质网状部神经元自发放电影响
Effect of Exendin-4 on the spontaneous discharge of neurons in the substantia nigra pars reticulata of mice
摘要
Abstract
Objective To investigate the effectof Exendin-4,a glucagon-like peptide-1(GLP-1)receptor agonist,on the spontaneous discharge of neurons in the substantia nigra pars reticulate of mice.Methods Immunofluorescent staining was used to observe the expression of GLP-1receptor(GLP-1R)in the substantia nigra pars reticulata of mice.Extendin-4 10 μmol/L were injected onto the surface of neurons in the substantia nigra pars reticulate using in vivo electrophysiological microelectrodes to ob-serve the spontaneous discharge of non-dopaminergic neurons(assumed to be GABAergic neurons)in the substantia nigra pars re-ticulate.Results Immunofluorescent staining showed a high expression level of GLP-1R in the substantia nigra par reticulate of mice.As for the 13 neurons recorded in the substantia nigra par reticulata,Exendin-4 significantly reduced the spontaneous dis-charge rate of 9 neurons,with a significant reduction in discharge frequency from(18.62±3.19)Hz to(14.615±3.06)Hz(t=7.257,P＜0.05),and the discharge frequency was reduced by(21.85±7.72)％on average,which was significantly higher than the reduction rate of(1.15±9.67)％in the normal saline group(Z=2.282,P＜0.05).In the other 4 neurons,Exendin-4 had no signi-ficant effect on discharge frequency,which changed from(13.49±7.16)Hz to(13.54±7.45)Hz.Conclusion Exendin-4 can re-duce the spontaneous discharge frequency of some neurons in the substantia nigra pars reticulata.关键词
艾塞那肽/黑质/网状部/胰高血糖素样肽-1受体/电生理学/小鼠,近交C57BLKey words
exenatide/substantia nigra/pars reticulata/glucagon-like peptide-1 receptor/electrophysiology/mice,inbred C57BL分类
医药卫生引用本文复制引用
沈方帅,李筱雪,刘翠,陈心怡,薛雁,陈蕾..Exendin-4对小鼠黑质网状部神经元自发放电影响[J].青岛大学学报（医学版）,2024,60(4):491-495,5.基金项目
国家自然科学基金青年科学基金项目(32200939) （32200939）
山东省自然科学基金资助项目(ZR2022QC225,ZR2023MC072) （ZR2022QC225,ZR2023MC072）
泰山学者青年专家计划资助项目(tsqnz20230634) （tsqnz20230634）
