青岛大学学报（医学版）2024，Vol.60Issue(4)：496-500,5.DOI:10.11712/jms.2096-5532.2024.60.110
UCH-L1和GFAP对硬膜外血肿进展的预测价值
Value of ubiquitin C-terminal hydrolase-L1 and glial fibrillary acidic protein in predicting the progression of epidural hematoma
摘要
Abstract
Objective To investigate the value of ubiquitin C-terminal hydrolase-L1(UCH-L1)and glial fibrillary acidic pro-tein(GFAP)in predicting the progression of epidural hematoma(EDH).Methods Male New Zealand white rabbits were ran-domly divided into sham-operation group(SH group),EDH 0.1 mL group,EDH 0.2 mL group,and EDH 0.3 mL group.Blood samples were collected at 1,3,6,and 24 h after surgery,and enzyme-linked immunosorbent assay was used to measure the levels of UCH-L1 and GFAP in plasma.Neurobehavioral scoring and brain water content measurement were performed at 24 h after sur-gery,and immunohistochemistry was used to measure the expression of UCH-L1 and GFAP in cerebral cortex.Results Compared with the SH group,the three EDH groups had a significant reduction in neurobehavioral score(F=290.10,P＜0.001)and a significant increase in brain water content(F=33.64,P＜0.001).There were significant differences in UCH-L1 and GFAP in cerebral cortex between the four groups(F=45.90,118.03;P＜0.001).Compared with the SH group,the three EDH groups had a significantly higher level of UCH-L1 in plasma at 1,3,and 6 h after surgery(F=3.15-47.93,P＜0.001)and a significantly higher level of GFAP in plasma at each time point after surgery(F=29.17-332.00,P＜0.001).Conclusion An increase in EDH volume can promote the expression of UCH-L1 and GFAP in the injured cortex and upregulate the levels of UCH-L1 and GFAP in plasma.UCH-L1 and GFAP in cerebral cortex and plasma can be used as effective biomarkers for predicting the progres-sion of EDH.关键词
血肿,硬膜外,颅内/生物标记/泛素C末端水解酶-L1/神经胶质原纤维酸性蛋白质Key words
hematoma,epidural,cranial/biomarkers/ubiquitin C-terminal hydrolase-L1/glial fibrillary acidic protein分类
医药卫生引用本文复制引用
刘俊汝,张广宇,邓明铷,聂安格,袁海成..UCH-L1和GFAP对硬膜外血肿进展的预测价值[J].青岛大学学报（医学版）,2024,60(4):496-500,5.基金项目
国家自然科学基金资助项目(81673360) （81673360）
