青岛大学学报（医学版）2024，Vol.60Issue(4)：501-507,7.DOI:10.11712/jms.2096-5532.2024.60.134
YY1对胶质瘤细胞迁移能力的影响及其分子机制
Effect of Yin Yang 1 on the migration ability of gliomacells and its molecular mechanism
摘要
Abstract
Objective To investigate the effect of the transcription factor Yin Yang 1(YY1)on the migration of glioma cells and its molecular mechanism.Methods Human glioma U251 and U87 cells were cultured in vitro,and after the overexpression or knockdown of YY1,Transwell assay,cell scratch assay,and colony formation assay were used to observe the effect of YY1 overexpression or knockdown on the migration of glioma U251 and U87 cells.The Gene Transcription Regulation Database was used to predict the downstream targetgenes of YY1,and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of matrix metalloproteinase 15(MMP15),a downstream target gene of YY1.Dual-luciferase reporter assay was used to investigate the binding region between YY1 and MMP15 promoter.Transwell assay,cell scratch assay,and colony formation assay were used to investigate the effect of HA-YY1 overexpression and MMP15 knockdown on the migration of glioma U251 and U87 cells.Results Transwell assay showed that compared with the control group without HA-YY1 overex-pression,the HA-YY1 overexpression group had a significant increase in the migration ability of U251 and U87 cells(t=13.300,5.847;P＜0.05).Cell scratch assay showed that the HA-YY1 overexpression group had a significant reduction in scratch spacing(t=9.698,7.215;P＜0.05).Colony formation assay showed significant increases in the colony formation ability of U251 and U87 cells with HA-YY1 overexpression(t=5.871,5.095;P＜0.05).Dual-luciferase reporter assay showed that YY1 could directly bind to the promoter region of MMP15 and promote the expression of MMP15,while knockdown of MMP15 could inhibit cell migration promoted by YY1 overexpression.Conclusion YY1 promotes the migration of glioma cells through transcriptional activation of MMP15.关键词
胶质母细胞瘤/YY1转录因子/基质金属蛋白酶15/细胞运动Key words
glioblastoma/YY1 transcription factor/matrix metalloproteinase 15/cell movement分类
医药卫生引用本文复制引用
解冉冉,吕志民,赵高翔..YY1对胶质瘤细胞迁移能力的影响及其分子机制[J].青岛大学学报（医学版）,2024,60(4):501-507,7.基金项目
国家自然科学基金资助项目(82002445) （82002445）
山东省自然科学基金资助项目(ZR2020QC084) （ZR2020QC084）
