青岛大学学报（医学版）2024，Vol.60Issue(4)：508-512,5.DOI:10.11712/jms.2096-5532.2024.60.121
高尿酸血症小鼠模型构建及其对肠屏障损伤的影响
Establishment of a mouse model of hyperuricemia with intestinal barrier injury
摘要
Abstract
Objective To establish a stable mouse model of intestinal barrier injury induced by hyperuricemia.Methods The mice were given different concentrations of adenine(Ade)and potassium oxazinate(PO)by gavage,and serum uric acid,urea nitrogen,creatinine,and intestinal permeability were measured on days 3,7,14,and 21.The expression levels of tight junction proteins Occludin,ZO-1,and Claudin-1 in the jejunum,ileum,and colon were measured.Results The high concentrations of Ade and PO induced a significant increase in uric acid(F=25.453-518.039,P＜0.01)and a significant reduction in body weight(F=6.900-43.724,P＜0.05).Administration of 50 mg/kg Ade and 125 mg/kg PO by gavage for 7 consecutive days induced a significant increase in uric acid(P＜0.05),which continued to rise until day 21(P＜0.05),and there was also a significant increase in intestinal permeability(F=28.563-185.808,P＜0.05),which was positively correlated with the level of uric acid(r=0.876 9,P＜0.05).Compared with normal mice,the mice with hyperuricemia had significant reductions in the expression levels of Occludin and ZO-1 in the ileum and the colon(t=3.164,3.678;P＜0.05),as well as a significant reduction in the expression level of Clau-din-1 in the jejunum(t=2.670,P＜0.05).Conclusion Administration of 50 mg/kg Ade and 125 mg/kg PO by gavage for 7 consecutive days can establish a mouse model of hyperuricemia with intestinal barrier injury.关键词
高尿酸血症/肠/容积渗克分子浓度/疾病模型,动物Key words
hyperuricemia/intestines/osmolar concentration/disease models,animal分类
医药卫生引用本文复制引用
朱现菊,吕秋兰,胡鹤鸣..高尿酸血症小鼠模型构建及其对肠屏障损伤的影响[J].青岛大学学报（医学版）,2024,60(4):508-512,5.基金项目
国家自然科学基金资助项目(81901575) （81901575）
