解剖学杂志2024，Vol.47Issue(4)：314-320,7.DOI:10.3969/j.issn.1001-1633.2024.04.007
虫草素通过调控Dickkopf相关蛋白/β-catenin信号通路诱导自噬和凋亡抑制弥漫大B细胞淋巴瘤
Cordycepin induces autophagy and apoptosis through regulating Dickkopf-related protein 1/β-catenin signal pathway to inhibit diffuse large B cell lymphoma
摘要
Abstract
Objective:To investigate whether cordycepin could induce autophagy and apoptosis through regulating Dickkopf-related protein 1(Dkk1)/β-catenin signal to inhibit diffuse large B cell lymphoma(DLBCL).Methods:DLBCL SU-DHL-4 cells were selected for research.Cell viability was detected by MTT assay.DLBCL SU-DHL-4 cells were treated with different concentrations of cordycepin and divided into control group,cordycepin 20 µg/mL group,cordycepin 40 µg/mL group,and cordycepin 80 µg/mL group.SU-DHL-4 cells were transfected with si-DDK1 and divided into control group,cordycepin 80 µg/mL group,and cordycepin 80 µg/mL+si-DDK1 group.Cell apoptosis rate was detected by the flow cytometry.The number of microtubule-associated protein light chain 3(LC3)positive cells was detected by fluorescence immunocytochemistry.The protein relative expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved poly-ADP-ribose polymerase(PARP1),becline-1,autophagy-related protein 7(Atg7),LC3Ⅱ,DDK-1 and β-catenin were detected by Western blotting.Results:Compared with the control group,after intervention with 40 and 80 µg/mL cordycepin,the apoptosis rate of SU-DHL-4 cells was increased,and the positive expression of LC3+was risen significantly,and the relative protein expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved PARP1,becline-1,ATG7,LC3Ⅱ/LC3Ⅰ,and DDK-1 were all up-regulated while the relative protein expression of β-catenin was down-regulated.Compared with the cordycepin 80 µg/mL group,the relative protein expression of DDK-1 was down-regulated in the cordycepin 80 µg/mL+si-DDK1 group,while the relative protein expression of β-catenin was up-regulated.At the same time,SU-DHL-4 cell apoptosis rate was reduced,and the positive expression of LC3+was significantly reduced,and the relative protein expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved PARP1,becline-1,ATG7,and LC3Ⅱ/LC3Ⅰ were all down-regulated.Conclusion:Cordycepin can induce autophagy and apoptosis through regulating Dkk1/β-catenin signal to inhibit DLBCL.关键词
弥漫大B细胞淋巴瘤/虫草素/凋亡/自噬/Dickkopf相关蛋白1/β-catenin信号通路Key words
diffuse large B cell lymphoma/cordycepin/apoptosis/autophagy/Dickkopf-related protein 1/β-catenin signaling pathway分类
医药卫生引用本文复制引用
岳运霞,闫海山,马俊华,陈培乐,王松琪,李千..虫草素通过调控Dickkopf相关蛋白/β-catenin信号通路诱导自噬和凋亡抑制弥漫大B细胞淋巴瘤[J].解剖学杂志,2024,47(4):314-320,7.基金项目
河南省医学科技攻关计划(2018020369) （2018020369）
