解剖学杂志2024，Vol.47Issue(4)：321-327,338,8.DOI:10.3969/j.issn.1001-1633.2024.04.008
miR-126-5p靶向MAPK1对甲状腺癌顺铂耐药细胞增殖和侵袭的影响
Effect of miR-126-5p targeting MAPK1 on proliferation and invasion of thyroid cancer cisplatin-resistant cells
摘要
Abstract
Objective:To investigate the effect and mechanism of miR-126-5p on cisplatin resistance,proliferation and invasion of thyroid cancer cells.Methods:The expression of miR-126-5p and MAPK1 mRNA in the cells were detected by RT-qPCR.The cells were divided into control group,miR-126-5p mimic group,miR-NC group,miR-126-5p mimic+pc-MAPK1group,miR-126-5p mimic+pc-NC group.The cell cisplatin(CDDP)resistance and proliferation were measured by CCK-8 assay,and invasion was measured by Transwell,the targeting relationship was verified by the dual-luciferase reporter assay,and expression level of MAPK1 protein was detected by Western blotting.Results:Compared with normal tissues and cells,the mRNA level of miR-126-5p was significantly down-regulated in thyroid cancer tissues and cells.Compared with the thyroid cancer cell line TPC-1,the expression of miR-126-5p in thyroid cancer cisplatin-resistant cell line TPC-1/CDDP was significantly down-regulated.Either in TPC-1 cells or TPC-1/CDDP cells,compared with the control group,the IC50 value of CDDP,cell proliferation,and the number of invasive cells per visual field of the miR-126-5p mimic group cells were all significantly reduced.The miR-126-5p targeted the down-regulation of MAPK1.Overexpression of MAPK1 reversed the effect of miR-126-5p overexpression on the IC50 value of cell CDDP,cell proliferation and the number of invasive cells per field.Conclusion:The miR-126-5p overexpression enhances the cisplatin sensitivity of TPC-1 and TPC-1/CDDP cells by targeting the down-regulation of MAPK1,and inhibits cell proliferation and invasion.关键词
miR-126-5p/MAPK1/甲状腺癌/顺铂/增殖/侵袭Key words
miR-126-5p/MAPK1/thyroid cancer/cisplatin/proliferation/invasion分类
临床医学引用本文复制引用
李璐,孙弟波,李萍,李思宇,马海燕,张泽天..miR-126-5p靶向MAPK1对甲状腺癌顺铂耐药细胞增殖和侵袭的影响[J].解剖学杂志,2024,47(4):321-327,338,8.基金项目
2020年度河北省医学科学研究课题计划(20200500) （20200500）
