水产学报2024，Vol.48Issue(10)：51-59,9.DOI:10.11964/jfc.20231114218
溶解氧变化对菲律宾蛤仔鳃组织结构的影响
Effects of dissolved oxygen changes on the gill tissue structure of Ruditapes philippinarum
摘要
Abstract
The Ruditapes philippinarum mainly inhabits estuaries,inner bays and coastal mudflat.Affected by tides,rainfall and seasons,the dissolved oxygen in their living environment is prone to change,which will affect their growth,survival and metabolism.The gills of bivalves are both respiratory organs and feeding organs used for filtering food.In order to investigate the effect of dissolved oxygen changes on the gill tissue structure of R.philip-pinarum and provide parameters for their aquaculture management,we designed three modes of dissolved oxygen changes,namely,continuous maintenance of regular dissolved oxygen C treatment,regular dissolved oxygen-rapid hypoxia for 24 h-rapid reoxygenation for 4 h AHR treatment,and regular dissolved oxygen-slow hypoxia for 48 h-slow reoxygenation for 8 h CHR treatment.Then tissue sections and immunohistochemstry methods were used to analyze the effect of dissolved oxygen changes on the gill tissue structure.The results of tissue sectioning showed that dissolved oxygen changes would affect the morphology and structure of gill tissue in R.philippinarum.Hyp-oxia caused gill filaments to widen,surface epithelial cells to damage,cilia to fall off,the sponge like blood cavity tissue to become loose,gill lumen gaps to become larger,and there were cell fragments inside.Hypoxia reoxygen-ation significantly changed the morphology of the gill flap,and the damage to the tissue cells of the outer gill flap was more severe than that of the inner gill flap.Slow hypoxia for 48 h and slow reoxygenation for 8 h caused more severe damage to the gill tissue structure than quick hypoxia for 24 h and quick reoxygenation for 4 h.The immun-ohistochemical results showed that both rapid hypoxia for 24 h and slow hypoxia for 48 h increase the ROS level in the gill tissue,while reoxygenation only reduced the ROS level a little in the gill tissue.There was no signific-ant repair effect on the damage caused by hypoxia in a short period of time.The gill tissue cells damaged by slow hypoxia for 48 h and reoxygenation for 8 h were numerous and diffuse,with unclear nuclear cytoplasmic boundar-ies,the degree of damage to gill tissue cells was higher than that of rapid hypoxia for 24 h and reoxygenation for 4 h.In summary,this experiment found that both slow hypoxia for 48 h and rapid hypoxia for 24 h could cause dam-age to the cellular structure of the gill tissue.The damage to reoxygenated tissue cells could not be repaired in a short period of time.Slow hypoxia for 48 h caused more severe damage to gill tissue structure than fast hypoxia for 24 h.The tissue cell damage continues to worsen even after 8 hours of slow reoxygenation.The results of this study suggested that the management of R.philippinarum farming need to consider the stable control of dissolved oxygen,and efforts should be made to avoid changes in dissolved oxygen,especially the damage to the gill tissue of clams caused by long-term chronic hypoxia stress.This study provides parameters for dissolved oxygen control in the process of R.philippinarum aquaculture.关键词
菲律宾蛤仔/鳃/形态结构/低氧/活性氧Key words
Ruditapes philippinarum/gill/morphological structure/hypoxia/ROS分类
农业科技引用本文复制引用
周丽青,葛广玉,井浩,吴宙,孙秀俊,李家乐,吴彪,刘志鸿,杨金龙..溶解氧变化对菲律宾蛤仔鳃组织结构的影响[J].水产学报,2024,48(10):51-59,9.基金项目
中国水产科学研究院基本科研业务费(2023TD30) （2023TD30）
崂山实验室科技创新项目(LSKJ202203803) （LSKJ202203803）
中国水产科学研究院黄海水产研究所基本科研业务费(20603022022001) （20603022022001）
青岛市市南区科技计划(2022-2-026-ZH) （2022-2-026-ZH）
莆田市科技计划(2021NJJ002) Central Public-interest Scientific Institution Basal Research Fund,CAFS(2023TD30) （2021NJJ002）
Techno-logy Innovation Program of the Laoshan Laboratory(LSKJ202203803) （LSKJ202203803）
Central Public-interest Scientific Institu-tion Basal Research Fund,YSFRI,CAFS(20603022022001) （20603022022001）
Science and Technology Plan Project of Shinan Dis-trict,Qingdao(2022-2-026-ZH) （2022-2-026-ZH）
Putian Science and Technology Plan Project(2021NJJ002) （2021NJJ002）
