水产学报2024，Vol.48Issue(10)：128-136,9.DOI:10.11964/jfc.20220513505
克氏原螯虾胚胎离体孵化与抱卵孵化的效果评估
Effects evaluation of in vitro incubation and maternal incubation of Procambarus clarkii embryos
摘要
Abstract
The aquaculture of Procambarus clarkii has developed rapidly nationwide in recent years.According to statistics,the production of P.clarkii in 2021 reached 2 633 600 tons,become the most highly-produced freshwa-ter crustaceans in China.However,limited knowledge about intensive breeding of P.clarkii seedlings has hindered its sustainable development.Artificial incubation may be an effective way to realize intensive breeding of P.clarkii seedlings.The mortality,hatching rate and embryo development of P.clarkii embryos were compared between in vitro incubation mode and maternal incubation mode to evaluate the feasibility of in vitro incubation of P.clarkii embryos and explore its artificial intensive incubation techniques.The results showed that the mortality of embryos at different incubation periods(2-4 days,5-7 days,8-10 days,11-13 days of the experiment)were sig-nificantly different.Embryos mortality in the prepared hatching stage(11-13 days of the experiment)was signific-antly higher than that in 2-4 days and 8-10 days,but there were no significant differences in total embryos mortal-ity between the two incubation treatments.At the same measurement time,the developmental stages of embryos of two incubation treatments were similar and there were no embryo morphological abnormalities.However,embryos hatched under in vitro incubation were susceptible to mycelial attachment,and the dead embryos of in vitro incub-ation were all infected by the mycelium.Two incubation treatments appeared the first instar larvae at the same time.The hatching rate of embryos of in vitro incubation was significantly lower than that of maternal incubation.But a higher hatching rate(76.91％±4.62％)of embryos under in vitro incubation was obtained.The study indic-ated that the feasibility of P.clarkii embryos under in vitro incubation was good.The main problem of embryos under in vitro incubation was the embryonic death due to the oomycete infection.In order to reduce the mortality of embryos under in vitro incubation,gradient anti-oomycete could be carried out.关键词
克氏原螯虾/离体孵化/抱卵孵化/胚胎发育Key words
Procambarus clarkii/in vitro incubation/maternal incubation/embryonic development分类
生物科学引用本文复制引用
覃丽蓉,李为,荣宽,张堂林,刘家寿..克氏原螯虾胚胎离体孵化与抱卵孵化的效果评估[J].水产学报,2024,48(10):128-136,9.基金项目
国家重点研发计划(2020YFD0900303,2019YFD0900304) （2020YFD0900303,2019YFD0900304）
湖北省技术创新专项重大项目(2018ABA102) （2018ABA102）
中国科学院青年创新促进会人才项目(2019331) National Key Research and Development Program of China(2020YFD0900303,2019YFD0900304) （2019331）
Technical Innovation Special Major Project of Hubei Province(2018ABA102) （2018ABA102）
Talent Project of Youth Innovation Promotion Association of Chinese Academy of Sciences(2019331) （2019331）
