药学研究2024，Vol.43Issue(9)：840-845,902,7.DOI:10.13506/j.cnki.jpr.2024.09.002
广藿香醇在小肠缺血再灌注损伤中的肠道保护作用分析
Protection effects of patchouli alcohol on the intestine ischemia reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effect of patchouli alcohol,which is the principal ingredient of Lingnan genuine medicinal herb Pogostemon cablin(Blanco)Benth.on the intestine ischemia reperfusion(IIR)injury in rats,so as to explore the new medicinal value of Pogostemon cablin(Blanco)Benth.Methods Rats were randomly divided into sham-operated group,model group,low dose,middle dose and high dose groups of Patchouli alcohol(PA).After oral administration of PA in each PA dose groups for 7 days,intestine ischemia reperfusion model was established by ligating su-perior mesenteric artery and reperfusion.The sham-operated group underwent sham-operation.After 4 hours reperfusion,the concentration of TNF-α,IL-6,LPS,SOD,MDA in small intestine were detected,the histopathological examination was scored according to the Chiu 6 grading method and the apoptosis index was calculated by TUNEL staining.Results Com-pared with the sham-operated group,the concentration of TNF-α,IL-6,LPS,SOD and MDA in model group elevated sig-nificantly(P＜0.05 or 0.01),additionally,histopathological score and apoptosis index also elevated obviously(P＜0.01).Af-ter treated with PA,the tissue lesions of small intestine were improved.The low and high dose groups of PA could conspicu-ously reduce the concentration of TNF-α,SOD and MDA.Moreover,the high dose group could suppress the IL-6 secretion and the cell apoptosis.However,in terms of LPS reduction,PA didn't have a significant effect.Conclusion It has been pre-liminarily found that PA can protect the intestine by inhibiting the cell apoptosis caused by IIR injury,reducing the oxidative stress and suppressing the production of inflammatory markers.关键词
小肠缺血再灌注损伤/广藿香醇/氧化应激/炎症因子/细胞凋亡Key words
Intestine ischemia reperfusion/Patchouli alcohol/Oxidative stress/Inflammatory factor/Cell apoptosis分类
中医学引用本文复制引用
冯学轩,潘晓慧,姚欣伶,刘月姝,张朋辉..广藿香醇在小肠缺血再灌注损伤中的肠道保护作用分析[J].药学研究,2024,43(9):840-845,902,7.基金项目
广东省中医药局科研项目(No.20211056) （No.20211056）
