药学研究2024，Vol.43Issue(9)：862-866,5.DOI:10.13506/j.cnki.jpr.2024.09.005
维吾尔族习用药神香草特征图谱与含量测定
Establishment of specific chromatogram of Uyghur traditional medicine Herba Hyssopi and determination of two constituents
摘要
Abstract
Objective To establish the HPLC specific chromatogram of Herba Hyssopi and to determine the contents of diosmin and linarin.Methods The analysis was performed on C18 column(4.6 mmx250 mm,5 μm),mobile phase was acetonitrile-0.1％phosphoric acid solution in a gradient elution manner at a flow rate of 1.0 mL·min-1,and detection wave-lengths were set at 328 nm for specific chromatogram,and 333 nm for diosmin and linarin.Results There were eighteen main chromatographic peaks in the HPLC specific chromatogram of Hyssopus Officinalis.Diosmin and linarin showed good linear relationships within the ranges of 10.773～430.92 ng and 0.324 7～324.73 ng,whose average recoveries(n=9)were 97.1％and 100.2％with the RSD of 1.5％and 0.7％,respectively.Conclusion This method was accurate and stable,which can be used for the quality control of Hyssopus Officinalis.关键词
高效液相色谱/维药/神香草/地奥司明/蒙花苷/特征图谱/相对保留时间Key words
HPLC/Uyghur medicine/Herba Hyssopi/Diosmin/Linarin/Specific chromatogram/Relative retention time分类
药学引用本文复制引用
苏建,朱靖,孔亚萍,尹璇,赵振霞,刘永利..维吾尔族习用药神香草特征图谱与含量测定[J].药学研究,2024,43(9):862-866,5.基金项目
河北省高层次人才资助项目(No.C20221109) （No.C20221109）
