四川林业科技2024，Vol.45Issue(5)：49-57,9.DOI:10.12172/202404230001
不同光照强度对油樟1,8-桉叶素含量的影响
Influence of different light intensities on 1,8-cineole content in Camphora longepaniculata leaves
摘要
Abstract
Camphora longepaniculata is a plant belonging to the Lauraceae family,and it is famous for its essential oil which is rich in 1,8-cineole.The 1,8-cineole has extensive economic value in the fields of food,chemical industry,and medical treatment.Plant growth process and secondary metabolism are regulated by changes in external environment,but there is no report on how light intensity affects the essential oil content and its composition changes,and how to regulate the expression of essential oil synthesis-related genes.In this experiment,C.longepaniculata leaves were used as materials to study the differences in the content of essential oil and 1,8-cineole in C.longepaniculata leaves under different light intensities,and to analyze the expression changes of eight key genes in the synthesis pathway of 1,8-cineole.The results showed that:(1)Different light intensities affected the leaf morphological development and endogenous hormone content;(2)There were significant differences in the content of essential oils and 1,8-cineole in leaves of C.longepaniculata under different lighting conditions.Light intensity was beneficial to the expression of key enzyme genes in the terpenoid synthesis pathway;(3)The expression of DXR,CMK,HGMR,GPPS,NPPS,CINS,and LIS were up-regulated in sun-side leaves of C.longepaniculata,while HDS were down-regulated.The purpose of this study was to deeply analyze the effect of light on the content of essential oil and 1,8-cineole in C.longepaniculata,and to provide a theoretical basis for further optimizing the cultivation conditions of C.longepaniculata,increasing the content of 1,8-cineole and regulating oil production by biotechnology.关键词
光照强度/油樟精油/1,8-桉叶素/萜类合成基因Key words
light intensity/essential oil/1,8-cineole/terpenoid synthesis gene引用本文复制引用
陈楠,柳欣,魏琴,赵鑫..不同光照强度对油樟1,8-桉叶素含量的影响[J].四川林业科技,2024,45(5):49-57,9.基金项目
基于多组学的油樟产油性能分析及其调控路径研究(412-0219019402) （412-0219019402）
