农业科学研究2024,Vol.45Issue(3):26-33,8.
超敏蛋白与壳寡糖对微咸水灌溉下番茄幼苗的影响
Effects of Hypersensitive Protein and Chitosan Oligosaccharide on the Growth,Photosynthesis,and Antioxidant Capacity of Tomato Seedlings under Brackish Water Irrigation
摘要
Abstract
This study aims to explore methods for mitigating salt damage during seedling growth in brackish water and to rationally utilize hypersensitive protein(HP)and chitosan oligosaccharide(COS).The tomato variety Yulinda was used as the test material,employing three treatment stages:seed soaking,leaf spraying,and irrigation in a greenhouse seedling tray.Eight experimental treatments were established:CK1:full-stage freshwater;CK2:full-stage brackish water(EC=2.5 g/L);A1:freshwater+500 times HP+brackish water;A2:freshwater+800 times HP+brackish water;B1:200 times HP+800 times HP+brackish water;B2:400 times HP+500 times HP+brackish water;C1:200 times HP+800 times COS+brackish water;C2:400 times HP+500 times COS+brackish water.The objective was to determine the optimal concentrations and application methods for HP and COS.Results demonstrated that under brackish water irrigation,soaking seeds in freshwater or HP prior to sowing,and spraying COS or HP during the seedling stage significantly improved the growth and development of tomato seedlings.Compared to the brackish water control(CK2),the root activity in A2 increased significantly by 277.15%.The net photosynthetic rate in B2 increased significantly by 34.96%,while the contents of malondialdehyde(MDA)and proline significantly decreased.Stomatal conductance in C2 increased significantly by 8.33%,and peroxidase and catalase activities increased significantly by 221.13%and 14.64%,respectively.Principal component analysis indicated that soaking seeds in 400 times HP before sowing and spraying 500 times COS at the seedling stage provided the optimal method for alleviating salt stress in tomato seedlings.This approach is recommended for cultivating robust seedlings under brackish water conditions.关键词
微咸水/番茄幼苗/超敏蛋白/壳寡糖/生长发育/光合作用/抗氧化能力Key words
brackish water/tomato seedling/hypersensitive protein/chitosan oligosaccharide/growth and development/photosynthesis/antioxidant capacity分类
农业科技引用本文复制引用
闫海静,冯玺,王嘉欣,李建设,高艳明..超敏蛋白与壳寡糖对微咸水灌溉下番茄幼苗的影响[J].农业科学研究,2024,45(3):26-33,8.基金项目
宁夏回族自治区中央引导地方科技发展专项"设施蔬菜高产优质绿色栽培关键技术集成创新与转化"(2021FRD05018) (2021FRD05018)