农业科学研究2024，Vol.45Issue(3)：62-69,8.
益生菌复合发酵沙棘酸奶的工艺优化及其体外抗氧化活性研究
Study on the Process Optimization and In Vitro Antioxidant Activity of Probiotic-Fermented Sea-Buckthorn Yogurt
摘要
Abstract
Using sea-buckthorn puree and milk as the main raw materials,a flavorful sea-buckthorn yogurt was developed through co-fermentation with a commercial starter culture and laboratory-preserved Lactobacillus plantarum YHG-87.The results of the univariate tests were comprehensively evaluated using sensory scores,DPPH free radical scavenging rates,and titration acidity.Additionally,the Plackett-Burman test and response surface methodology were conducted using sensory scores as the response variable to determine the optimal preparation parameters and further investigate its in vitro antioxidant activity.The results indicated that the prepared sea-buckthorn yogurt had a viscous texture,delicate taste,and well-balanced flavor,featuring the unique aroma of sea-buckthorn and the rich scent of yogurt,under the conditions of a strain ratio of commercial bacteria to Lactobacillus plantarum YHG-87(m/m)of 1∶2,with 6%sea-buckthorn juice,7%sucrose,an inoculation level of 0.11%,and fermentation at 42.0℃for 6 hours.Under these conditions,the total phenolic content of the developed sea-buckthorn yogurt was(23.94±0.34)mg/100 g,the DPPH free radical scavenging capacity was(30.01±0.59)mg/100 g,the ABTS free radical scavenging capacity was(85.82±1.70)mg/100 g,and the iron ion reduction capacity was(24.90±0.39)mg/100 g,all of which were significantly higher than those of the control group.This study provides a theoretical basis for the development of antioxidant-rich sea-buckthorn yogurt and offers a novel approach to utilizing sea-buckthorn resources.关键词
乳酸菌/沙棘酸奶/最佳工艺/体外抗氧化活性Key words
lactic acid bacteria/sea-buckthorn yogurt/optimal craftsmanship/in vitro antioxidant activity分类
轻工业引用本文复制引用
丁雨红,剧柠,李亚凤,朱晓雪,杨秉坤,庄姣..益生菌复合发酵沙棘酸奶的工艺优化及其体外抗氧化活性研究[J].农业科学研究,2024,45(3):62-69,8.基金项目
宁夏回族自治区重点研发计划项目(2021BEF02022) （2021BEF02022）
宁夏回族自治区自然科学基金项目(2022AAC03023) （2022AAC03023）
