陕西医学杂志2024，Vol.53Issue(10)：1416-1421,6.DOI:10.3969/j.issn.1000-7377.2024.10.026
长链非编码RNA 00941、微小RNA-145表达及转化生长因子β1/Smad通路相关因子在胃癌组织中的表达及意义
Expression and significance of Linc00941,miR-145 and TGF-β1/Smad pathway-related factors in gastric cancer
摘要
Abstract
Objective:To investigate the expression and significance of Linc00941,miR-145 and TGF-β1/Smad pathway related factors in gastric cancer tissues.Methods:A total of 51 cases of gastric cancer were selected,and the diagnosis was diagnosed after surgery.Gastric cancer tissues and adjacent tissues of patients were collected,and the expression differences of Linc00941,miR-145 and TGF-β1/Smad pathway related factors were compared.Gastric cancer tissue cells were extracted from patients for tissue culture,and transfected and silenced Linc00941 and miR-145 genes to analyze their effects on the proliferation,migration and invasion of gastric cancer cells.Results:Compared with paracancer tissues,the relative expression levels of Linc00941 and its mRNA were increased in patients with gastric cancer tissues,while the relative expression levels of miR-145 and its mRNA were decreased(all P＜0.05).The levels of TGF-β1,transforming growth factor β receptor Ⅱ(TGF-βR)and Smad2/3 in gastric cancer tissues were significantly lower than those in adjacent tissues(all P＜0.05).There were statistically significant differences in absorbance value(A value)and apoptosis rate among NC group,Linc00941 transfection group and Linc00941 si-lencing group(all P＜0.05).The A value of Linc00941 silenced group were lower than that of NC group at 24,48 and 72 hours,and the apoptosis rate was higher than that of NC group(all P＜0.05).The A value of Linc00941 transfection group was higher than that of NC group,and the apoptosis rate was lower than that of NC group(all P＜0.05).Compared with NC group,the number of cell migration and cell invasion decreased in Linc00941 silencing group,and increased in Linc00941 transfection group,and the pairings between groups were statistically significant(all P＜0.05).Compared with the NC group,the A value of miR-145 transfected group was decreased for 24,48 and 72 hours,and the apoptosis rate was increased(all P＜0.05);in the miR-145 silencing group,the A value was in-creased,and the apoptosis rate was decreased(all P＜0.05).Compared with the NC group,the number of cell migra-tion and cell invasion in the miR-145 transfection group were decreased,and the number of cell invasion in the miR-145 silencing group was increased,and the pair-to-pair comparison between the groups was statistically significant(all P＜0.05).Conclusion:In gastric cancer tissues,the relative expression levels of Linc00941 and its mRNA were increased,the relative expression levels of miR-145 and its mRNA were decreased,and the expressions of TGF-β1/Smad pathword-related factors TGF-β1,TGF-βR Ⅱ and Smad2/3 were decreased.Moreover,the expression of Linc00941 and miR-145 affected the proliferation,invasion and migration of gastric cancer cells.关键词
长链非编码RNA 0094/微小RNA-145/转化生长因子β1/Smad通路/胃癌/表达水平/增殖能力Key words
Linc00941/miRNA-145/TGF-β1/Smad pathway/Gastric cancer/Expression level/Proliferative ability分类
医药卫生引用本文复制引用
法良玲,孙伟,黄臻..长链非编码RNA 00941、微小RNA-145表达及转化生长因子β1/Smad通路相关因子在胃癌组织中的表达及意义[J].陕西医学杂志,2024,53(10):1416-1421,6.基金项目
山东省医药卫生科技发展计划项目(202109010314) （202109010314）
