山东农业科学2024，Vol.56Issue(9)：1-5,5.DOI:10.14083/j.issn.1001-4942.2024.09.001
利用CRISPR/Cas9基因编辑片段敲除技术创制玉米多样化等位变异
Engineering Diversified Allelic Variation in Maize by Fragment Knockout Using CRISPR/Cas9 Genome Editing
摘要
Abstract
Engineering allelic variation by CRISPR/Cas9 gene editing technology has become an impor-tant mean to increase germplasm resources diversity in many plants.Increasing the content of carotenoids in ce-reals such as maize is an economic approach to solve vitamin A deficiency in developing countries.In this stud-y,the crtRB1 gene regulating carotenoid synthesis in maize was chosen as subject,the CRISPR/Cas9 dual-target system was used to perform fragment deletion on the promoters of crtRB1 in maize protoplasts,and then new diversified allelic variations with fragment deletion and InDel(insertion or deletion)were engineered.The results laid a foundation for the engineering of mutations in crtRB1 promoters and was of great significance to the functional research of promoter elements and improvement of maize quality.关键词
玉米/crtRB1/基因编辑/片段敲除/启动子Key words
Maize/crtRB1/Gene editing/Fragment knockout/Promoter分类
生物科学引用本文复制引用
张茂林,刘强,汪黎明,曾廷儒,王娟,严佳丽,何春梅,徐倩,刘铁山,董瑞,刘春晓,关海英..利用CRISPR/Cas9基因编辑片段敲除技术创制玉米多样化等位变异[J].山东农业科学,2024,56(9):1-5,5.基金项目
山东省自然科学基金面上项目(ZR2021MC084) （ZR2021MC084）
农业生物育种重大项目"黄淮海饲用高产玉米种质创制与新品种培育"(2023ZD0403002) （2023ZD0403002）
