生态学报2024，Vol.44Issue(18)：8208-8218,11.DOI:10.20103/j.stxb.202309041908
夏季水涝对白皮松根系电阻抗特性及生长生理的影响
Effects of waterlogging in summer on electrical impedance characteristics,growth and physiology of roots in Pinus bungeana seedlings
摘要
Abstract
Extreme precipitation in summer occurs more frequently in the context of climate change.Waterlogging in summer affects the growth and survival of trees by affecting their roots,thus causing serious damage to forest ecology.Since tree roots are buried underground,changes of roots after waterlogging are not easy to observe.The aim of this study was to use electrical impedance spectrum(EIS)method to non-destructively measure the electrical impedance characteristics of roots,and therefore providing a new method for quickly understanding the damage degree of underground roots after waterlogging stress by analyzing the correlation between electrical impedance parameters and root growth and physiological indexes.In the study,four-year-old lacebark pine(Pinus bungeana Zucc.)seedlings,commonly used in afforestation in north China,were exposed to waterlogging treatments in summer:ⅰ)Control(CK),normal management;ⅱ)Continuous waterlogging for 3 weeks(CWL);and ⅲ)Intermittent waterlogging for 3 weeks(IWL,waterlogging of 1 week+drainage of 1 week,a total of 3 cycles,cumulative waterlogging for 3 weeks).After waterlogging and during the subsequent recovery growth period,the EIS of the root system were measured and the spectra were classified by Class-Featuring Information Compression(CLAFIC).Morphological indexes,non-structural carbohydrates,and malondialdehyde content of roots were measured and their correlations with the EIS parameters were analyzed.Root morphological indexes in CWL and IWL treatments were lower than that in CK,and the root morphological indexes in IWL treatment were significantly lower than that in CK and CWL treatment during the recovery period.The results indicated that both continuous and intermittent waterlogging for 3 weeks inhibited the root growth,and intermittent waterlogging had more serious damage to roots than continuous waterlogging,which were confirmed by the higher content of malondialdehyde in roots.The EIS of the roots showed clear changes in CWL and IWL treatments,therein,the resistance value(Re(Z))was lower,the reactance value(Im(Z))was higher than that in CK,and the above parameters during the recovery growth stage changed in the opposite direction.The CLAFIC classification results showed that EIS of roots in CWL and IWL had the highest similarity during the waterlogging period,and their similarity with CK was higher after one month of recovery.The impedance loss factor(δ)at low frequency was linearly and positively correlated with root volume,whereas it was negatively correlated with starch content.The results suggest that impedance loss factor has the potential to characterize the root growth and physiological changes of lacebark pine seedlings after waterlogging stress.关键词
电阻抗/非结构性碳水化合物/白皮松/根系/水涝胁迫Key words
electrical impedance spectra/non-structural carbohydrate/Pinus bungeana Zucc./root system/waterlogging stress引用本文复制引用
刘召慧,崔叶红,郑芳,刘祎明,刘艳,盖雨兰,王爱芳..夏季水涝对白皮松根系电阻抗特性及生长生理的影响[J].生态学报,2024,44(18):8208-8218,11.基金项目
国家自然科学基金(青年科学基金项目)(32101250) （青年科学基金项目）
河北省省属高等学校基本科研业务费(KY2022004) （KY2022004）
