特产研究2024，Vol.46Issue(5)：78-81,4.DOI:10.16720/j.cnki.tcyj.2024.162
HPLC-DAD法测定铁筷子中香草酸和阿魏酸的含量
Determination of Vanillic Acid and Ferulic Acid in Iron Chopstick by HPLC-DAD Method
摘要
Abstract
A high performance liquid chromatography(HPLC)-Diode Array Detector(DAD)method was established for the determina-tion of vanillic acid and ferulic acid in iron chopsticks herbs.The chromatography was performed on ZORBAX Eclipse Plus C18 column(4.6 mm× 250 mm,5 μm).The mobile phase was acetonitrile(A)-0.2％phosphoric acid water(B),gradient elution,flow rate 1.0 mL/min;column temperature 25℃;detection wavelength(0～15 min,280 nm,vanillic acid;15-70 min,327 nm,ferulic acid);the injection volume was 10 μL.The separation degree of vanillic acid and ferulic acid was more than 1.5,and the separation effect was good,and the linear rela-tionship was good in the range of 22.72～727.00 μg/mL and 9.69～310.00 μg/mL,respectively,with r＞0.999.The average recoveries(RSD)were 101.08％(3.09％)and 98.98％(3.11％),respectively.The contents of ferulic acid and vanillic acid in iron chopsticks herbs S1 were the highest,the vanillic acid content in S1 was(0.77±1.42)mg/g and ferulic acid content was(0.36±1.32)mg/g.This study established HPLC-DAD switching wavelength method is sensitive,accurate and reproducible,and can be used for the determination of vanillic acid and ferulic acid in the iron chopsticks herbs.关键词
铁筷子/香草酸/阿魏酸/HPLC-DAD波长切换法Key words
iron chopsticks herbs/vanillic acid/ferulic acid/HPLC-DAD switching wavelength method分类
医药卫生引用本文复制引用
郭瑛,李安林,贺元,冯倩倩,田莉..HPLC-DAD法测定铁筷子中香草酸和阿魏酸的含量[J].特产研究,2024,46(5):78-81,4.基金项目
新疆维吾尔自治区药品监督管理局科研项目(新药监20210019[科]) （新药监20210019[科]）
新疆维吾尔自治区"十四五"重点学科项目(中医学[1005]) （中医学[1005]）
