西北林学院学报2024，Vol.39Issue(5)：68-77,10.DOI:10.3969/j.issn.1001-7461.2024.05.09
陆均松和青梅根系细菌群落多样性特征及其影响因素
Diversity Characteristics and Influencing Factors of Root Bacterial Community of Dacrydium pectinatum and Vatica mangachapoi
摘要
Abstract
The objectives of this study were to further explore the relationship between root bacterial com-munity structure and environmental factors.Based on different niches,the structure,diversity and function of bacterial community were analyzed by Illumina Miseq high-throughput sequencing technique and by using the the roots of D.pectinatum and V.mangachapoi,two typical tropical rain forest plants occurring in Hainan Island.The results showed that 1)the α diversity of bacterial community in the root system of D.pectinatum and V.mangachapoi was significantly affected by niche,and the α diversity index of bacteri-al community in rhizosphere and rhizoplane was significantly different from that of endosphere,and α di-versity decreased along the order of rhizosphere,rhizoplane and endosphere.2)There were significant differences in root bacterial community structure in different niches.Niche explained 14.7％of the differ-ence in root bacterial community composition,while plant species explained 11.6％.At phylum level,the first five phylums with the highest relative abundance were Proteobacteria,Actinobacteriota,Acidobacteri-ota,Planctomycetota and Verrucomicrobiota.The main dominant bacteria in the root bacterial community were Acidothermus,norank_f__Xanthobacteracea,Mycobacterium and Acidibacter,norank_f__norank_o__Elsterales.3)The functional analysis showed that some functional groups of bacterial community were sig-nificantly different among different root niches,and rhizoplane enriched a large number of functional groups(chemoheterotrophy,aerobic_chemoheterotrophy,etc.).4)There was a significant correlation of bacterial communities between different root niches and different environmental factors.The main environ-mental factors driving the difference of bacterial structure between D.pectinatum and V.mangachapoi were temperature and soil pH.Compared with tree species,niche has a stronger effect on root bacterial community structure.Soil pH and TK(total potassium)were the most important environmental factors affecting root bacterial community structure and diversity,respectively.The results of this study provide a scientific basis for promoting the growth,development and ecological protection of endangered tree species D.pectinatum and V.mangachapoi in tropical rain forest.关键词
陆均松/青梅/根系/细菌群落/生态位/多样性Key words
Dacrydium pectinatum/Vatica mangachapoi/root/bacterial community/niche/diversity分类
农业科技引用本文复制引用
王欣,庞通,魏亚情,许心诺,李玉武,兰国玉..陆均松和青梅根系细菌群落多样性特征及其影响因素[J].西北林学院学报,2024,39(5):68-77,10.基金项目
海南省重点研发项目(ZDYF2024SHFZ096) （ZDYF2024SHFZ096）
国家自然科学基金(32271603) （32271603）
海南儋州热带农业生态系统国家野外科学观测研究站开放课题(RRI-KLOF202405) （RRI-KLOF202405）
现代农业产业技术体系建设专项(CARS-33-ZP3). （CARS-33-ZP3）
