陆均松和青梅根系细菌群落多样性特征及其影响因素OA北大核心CSTPCD

The objectives of this study were to further explore the relationship between root bacterial com-munity structure and environmental factors.Based on different niches,the structure,diversity and function of bacterial community were analyzed by Illumina Miseq high-throughput sequencing technique and by using the the roots of D.pectinatum and V.mangachapoi,two typical tropical rain forest plants occurring in Hainan Island.The results showed that 1)the α diversity of bacterial community in the root system of D.pectinatum and V.mangachapoi was significantly affected by niche,and the α diversity index of bacteri-al community in rhizosphere and rhizoplane was significantly different from that of endosphere,and α di-versity decreased along the order of rhizosphere,rhizoplane and endosphere.2)There were significant differences in root bacterial community structure in different niches.Niche explained 14.7％of the differ-ence in root bacterial community composition,while plant species explained 11.6％.At phylum level,the first five phylums with the highest relative abundance were Proteobacteria,Actinobacteriota,Acidobacteri-ota,Planctomycetota and Verrucomicrobiota.The main dominant bacteria in the root bacterial community were Acidothermus,norank_f__Xanthobacteracea,Mycobacterium and Acidibacter,norank_f__norank_o__Elsterales.3)The functional analysis showed that some functional groups of bacterial community were sig-nificantly different among different root niches,and rhizoplane enriched a large number of functional groups(chemoheterotrophy,aerobic_chemoheterotrophy,etc.).4)There was a significant correlation of bacterial communities between different root niches and different environmental factors.The main environ-mental factors driving the difference of bacterial structure between D.pectinatum and V.mangachapoi were temperature and soil pH.Compared with tree species,niche has a stronger effect on root bacterial community structure.Soil pH and TK(total potassium)were the most important environmental factors affecting root bacterial community structure and diversity,respectively.The results of this study provide a scientific basis for promoting the growth,development and ecological protection of endangered tree species D.pectinatum and V.mangachapoi in tropical rain forest.