橡胶树HbWRI转录因子的克隆与表达分析OA北大核心CSTPCD

Lipid is the main non-rubber component of Hevea brasiliensis,and it is closely related to latex metabolism and natural rubber quality formation.However,few reseaches were found on its metabolic reg-ulation.WRINKLED(WRI),a member of the plant AP2 transcription factor family,plays an important role in promoting the flow of carbon sources from sugar to lipid synthesis in plants.In this study,three transcription factors HbWRI1,HbWRI2 and HbWRI3 of the HbWRI gene family were successfully cloned from the latex of H.brasiliensis,respectively.And their bioinformatics analysis,expression analysis of different tissue sites and subcellular localization analysis were performed.The results showed that the encoding sequences of HbWRI1,HbWRI2 and HbWRI3 were 1 128,1 122 and 1 050 bp,encoding 375,373 and 349 amino acids.HbWRI1-3 protein has two AP2 conserved domains with 60-70 amino acids.The first AP2 domain contains a VYL motif and a 14-3-3 protein-binding motif.Bioinformatics analysis predic-ted that HbWRI1-3 protein had no transmembrane domains,no signal peptide,hydrophobic protein and multiple phosphorylation sites.Subcellular localization analysis showed that these protein were located in the nucleus.The expression level of HbWRI1,HbWRI2 and HbWRI3 were the highest in latex.The ex-pression level of HbWRI3 gene in latex was the highest in HbWRI1-3.It is speculated that HbWRI1-3 may be involved in the regulation of latex metabolism such as lipid and rubber biosynthesis.This study lays a foundation for further exploring the effects of HbWRI1-3 on the regulation of latex metabolism and the quality of natural rubber.