长链非编码RNA LOC102639982和LOC102636309与自身免疫性肝炎肝损伤的关联OACSTPCD

Objective To investigate the biological mechanisms of differentially expressed long non-coding RNA(lncRNA)LOC102639982 and lncRNA LOC102636309 in the development of autoimmune hepatitis(AIH).Methods Twelve SPF grade female C57BL/6 mice were randomly divided into normal group and model group.The mice in model group were injected with concanavalin A(ConA)in the tail vein to prepare the mouse model of AIH,and the mice in normal group were injected with normal saline.The liver and serum of mice in each group were collected.The necrosis degree of hepatocytes and the infiltration degree of liver inflammatory cells were observed by HE staining.The contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),nitric oxide(NO)and malondialdehyde(MDA)in serum were detected by ELISA.The expression levels of lncRNA LOC102639982 and lncRNA LOC102636309 in liver were detected by qRT-PCR,GO and KEGG enrichment analysis and lncRNA-transcription factor association analysis were performed on the mRNA co-expressed by the two lncRNAs.Results HE staining showed that the hepatocytes in model group were degenerated and necrotic,with a large number of infiltrated inflammatory cells.Compared with normal group,the levels of NO and MDA in liver tissues and the serum ALT and AST levels were significantly increased in model group(P<0.01).Changes of lncRNA LOC102639982 and lncRNA LOC102636309 expression by qRT-PCR were consistent with the results of microarray chip.The results of qRT-PCR combined with the results of liver injury showed that the change trend of microarray chip results was consistent with that of liver injury indexes.The bioinformatics results showed that the mRNAs co-expressed by lncRNA LOC102639982 and lncRNA LOC102636309 were mainly enriched in protein binding in molecular function,and mainly enriched in transcriptional regula-tion and DNA templateization in biological processes.The mRNAs co-expressed with lncRNA LOC102639982 were mainly enriched in cytokine and cytokine receptor interaction and TNF signaling pathways,while the mRNAs co-expressed with lncRNA LOC102636309 were mainly enriched in MAPK and NF-kappa B signaling pathways.LncRNA LOC102639982 was most strongly associated with the transcription factors NR4A1,ATF3,while lncRNA LOC102636309 was most strongly associated with the transcription factor Fols2.Conclusion The differential expressions of lncRNA LOC102639982 and lncRNA LOC102636309 are strongly correlated with autoim-mune hepatitis liver injury,and both may be potential targets for the prevention and treatment of autoimmune hepatitis.