土壤2024，Vol.56Issue(4)：826-832,7.DOI:10.13758/j.cnki.tr.2024.04.016
外源Ca2+对两种产脲酶细菌修复Cd-As复合污染水稻土的影响
Effect of Exogenous Ca2+ on Remediation of Cd-As Co-contaminated Paddy Soil by Two Urease-producing Bacteria
摘要
Abstract
Microbial-induced carbonate precipitation(MICP)has been widely used in the remediation of heavy metal contaminated soil.Two urease-producing bacteria,Octococcus pasteurii and Bacillus cereus,with or without exogenous Ca2+were introduced to Cd-As co-contaminated paddy soil in order to optimize the process of MICP and improve remediation efficiency.The impact of exogenous CaCl2 on the efficacy of Cd and As remediation in the soil under two strains treatments,as well as the changes of soil properties,enzyme activities and microbial diversity after remediation were studied.Results showed that two strains were able to immobilize Cd and As in soil,compared with Octococcus pasteurii,Bacillus cereus reduced available Cd and As contents by 16.7％and 11.1％,respectively.The addition of exogenous Ca2+reduced available Cd and As by 17.3％-22.2％and 16.8％-26.7％in both bacterial treatments,respectively,indicating that the addition of Ca2+accelerated MICP process and immobilized Cd and As.Compared with the treatment without Ca2+,the addition of Ca2+significantly increased soil urease(52.6％-113.3％),sucrase activity(13.1％-28.9％),alkaline hydrolysis nitrogen(3.4％-25.5％),available potassium(2.1％-34.1％)and microbial diversities in both bacterial treated soils,implying that the addition of exogenous Ca2+effectively enhanced soil fertility and soil ecological functions.In conclusion,the remediation efficiency of Cd-As co-contaminated soil by urease producing bacteria can be improved by the addition of Ca2+,with Bacillus cereus offering a higher efficiency for reducing the phytoavailability of heavy metals in soil.关键词
微生物诱导碳酸盐沉淀(MICP)/外源Ca2+/产脲酶细菌/土壤修复Key words
Microbial induced carbonate precipitation/Ca2+from exogenous sources/Urease-producing bacteria/Soil remediation分类
资源环境引用本文复制引用
王金坪,费藏乐,邵梦迪,杨润,罗燕,黄金富,蔡倩,伍钧,徐敏..外源Ca2+对两种产脲酶细菌修复Cd-As复合污染水稻土的影响[J].土壤,2024,56(4):826-832,7.基金项目
国家自然科学基金项目(42107017)、成都市科技项目(2020-YF09-00023-SN和2020-YF09-00012-SN)和大学生创新训练计划项目资助. （42107017）
