陕西医学杂志2024，Vol.53Issue(10)：1309-1313,5.DOI:10.3969/j.issn.1000-7377.2024.10.003
α-干扰素联合索拉菲尼对肝癌细胞增殖的抑制作用及其对STAT3通路的影响
Inhibitory effect of α-interferon combined with sorafenib on proliferation of hepatocellular carcinoma cells and its effect on STAT3 pathway
摘要
Abstract
Objective:To investigate the inhibitory effect of α-interferon(IFN-α)combined with sorafenib on the proliferation of hepatocellular carcinoma cell and its effect on transcriptional activator 3(STAT3).Methods:Human hepatocellular carcinoma cell line HepG2 was selected and divided into blank control group(HL-60 cells without any treatment),IFN-α group(adding 4000 U/ml IFN-α),sorafenib group(adding 2 μmol/L sorafenib),IFN-α+sor-afenib group(adding 4000 U/ml and 2 μmol/L sorafenib).Cell suspension of each group after drug treatment was taken and cultured for 24,48 and 72 hours.The survival rate of HepG2 cells was detected by CCK8 and the prolifera-tion inhibition rate of HepG2 cells was detected by MTT method.The expressions of STAT3 and Survivin mRNA and protein in HepG2 cells were measured by Western Blot and qRT-PCR,and the cell morphology was observed.Results:In the blank control group,the cells were in a complete shape,with clear outline,find-shaped,and well adher-ent state;in the IFN-α group and sorafenib group,part of the cytoplasm was swollen,the cell membrane was broken,and the cell morphology and membrane boundaries were blurred;in the IFN-α+sorafenib group,the nucleus was re-duced,chromatin was contracted,and some cells were broken and died.The cell proliferation inhibition rate in IFN-α+sorafenib group was higher than that in IFN-α and sorafenib groups(all P＜0.05).Compared with blank control group,IFN-α group and sorafenib group,cell organelles of IFN-α+sorafenib group increased in G1 phase,and cells of S and G2 phase decreased(all P＜0.05).Compared with blank control group,STAT3,Survivin mRNA and protein expressions in IFN-α group,sorafenib group and IFN-α+sorafenib group were decreased(all P＜0.05),but there was no significant difference between IFN-α group and sorafenib group(all P＞0.05),and IFN-α+sorafenib group was lower than those of the other three groups(all P＜0.05).Conclusion:α-interferon combined with sorafenib can inhib-it proliferation and induce apoptosis of HepG2 cells,possibly by regulating STAT3 pathway.关键词
α-干扰素/索拉菲尼/肝癌/细胞增殖/凋亡/STAT3信号通路Key words
Alpha-interferon/Sorafenib/Liver cancer/Cell proliferation/Apoptosis/STAT3 signaling pathway分类
医药卫生引用本文复制引用
胡薪蕊,吴熙,殷玉琨,胡刚,冯利..α-干扰素联合索拉菲尼对肝癌细胞增殖的抑制作用及其对STAT3通路的影响[J].陕西医学杂志,2024,53(10):1309-1313,5.基金项目
北京市重大疑难疾病中西医协同攻关项目(2023BJSYNZDJBXTGG-004) （2023BJSYNZDJBXTGG-004）
