陕西科技大学学报2024，Vol.42Issue(5)：67-76,10.
槟榔次碱速效抗疲劳活性及其通过调控血脑屏障发挥抗疲劳机制研究
Study on the quick-acting antifatigue activity of arecaidine and its mechanism of anti-fatigue by regulating the blood brain barrier
摘要
Abstract
To explore the development prospects of arecaidine anti fatigue products,a mouse behavioral sensitization model was constructed to analyze the quick-acting anti fatigue activi-ty and its mechanism of arecaidine by using indicators such as the exhaustive swimming time,blood lactate,hemoglobin(Hb),blood urea nitrogen,malondialdehyde,muscle glycogen,liver glycogen,Evans blue,5-hydroxytryptamine(5-HT),dopamine(DA)and 5-HT/DA value.The results showed that arecaidine could prolong the exhaustive swimming time of mice,and behavioral sensitization could effectively enhance the activity of arecaidine.The exhaustive swimming time of both treatments of behavioral sensitized mice was significantly longer than that of two non sensitized treatments and two controls(P＜0.01).Oral sustained-release ad-ministration also promoted the activity of arecaidine.Arecaidine can inhibit the accumulation of blood lactate,malondialdehyde and blood urea nitrogen in swimming mice,and alleviate the decrease in Hb and DA levels,and weaken the increase of 5-HT in the brain,and inhibit the depletion of muscle glycogen and liver glycogen,and hinder the increase of 5-HT/DA values in the brain,and enhance the permeability of the blood-brain barrier(BBB),Moreover,and behavioral sensitization and oral sustained-release administration can enhance the above effects.Arecaidine can exert anti fatigue effects by inhibiting the decrease of oxygen transport protein levels,balancing central neurotransmitters,reducing the accumulation of metabolites and energy consumption,regulating BBB permeability to allow 5-HT in the brain to diffuse into the bloodstream,and reducing the inhibition of 5-HT on brain excitation.Arecaidine can only exert excellent quick-acting anti fatigue activity on behavior sensitized organisms through oral sustained-release administration,the reason may be that arecaidine is absorbed through the oral mucosa,avoiding the first pass effect of the gastrointestinal tract and rapidly increasing its entry into the bloodstream.This study can provide theoretical support for the development of quick-acting anti fatigue products of arecaidine.关键词
槟榔次碱/血脑屏障/速效抗疲劳/神经递质/行为敏化Key words
arecaidine/blood-brain barrier/quick-acting anti-fatigue/neurotransmitter/be-havioral sensitization分类
医药卫生引用本文复制引用
刘书伟,张田田,王燕,武天明..槟榔次碱速效抗疲劳活性及其通过调控血脑屏障发挥抗疲劳机制研究[J].陕西科技大学学报,2024,42(5):67-76,10.基金项目
海南省科技专项项目-海南省重点研发项目(ZDYF2022XDNY172) （ZDYF2022XDNY172）
海南省自然科学基金项目(321RC589,221MS049,423MS052) （321RC589,221MS049,423MS052）
