刑事技术2024，Vol.49Issue(5)：514-518,5.DOI:10.16467/j.1008-3650.2023.0073
陈旧颅骨的DNA检验
DNA Test of Aged Skull
摘要
Abstract
DNA test of aged skull is always a difficult problem in the field of forensic science,as the skull contains little DNA,and the DNA degrades badly.Here,an improved method is introduced for DNA test of aged skulls.Taking the skulls in recent cases for example,the optimization was carried out from the selection of materials,concentration and recovery of demineralized solution,high volume extraction and purification of DNA,etc.Finally,the results of autosomal and Y chromosome STR polymorphism were obtained successfully.The results showed that petrosal part of temporal bone could be the preferred extraction site for the test of aged skulls,with a higher success rate compared with other parts of the skull;in addition,the use of Amicon Ultra-15 10K centrifugal ultrafiltration tubes could remove a large number of small molecules,such as water and ions in the decalcification solution,so as to retain and recycle the large molecules of DNA,which effectively reduced the amount of DNA loss;furthermore,the quality of DNA was poor in aged skulls,so increasing the amount of bone powder used and increasing the extraction and purification system could also significantly improve the amount of DNA recovered.This method improves the quantity and quality of DNA recovered from aged skull,and can provide reference for the follow-up DNA test of similar aged bones and teeth.关键词
法医物证学/陈旧颅骨/颞骨岩部/DNA提取/脱钙液回收Key words
forensic genetics/aged skull/petrosal part of temporal bone/DNA extraction/recovery of demineralized solution分类
社会科学引用本文复制引用
李永久,张广峰,窦雪丽,刘洪迪,彭柱,刘志芳,凃政..陈旧颅骨的DNA检验[J].刑事技术,2024,49(5):514-518,5.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2018JB012,2020JB009) （2018JB012,2020JB009）
公安部科技强警基础工作专项计划(2021JC14) （2021JC14）
