针刺研究2024，Vol.49Issue(10)：1001-1009,9.DOI:10.13702/j.1000-0607.20230337
针刺对卵巢低反应小鼠卵巢的保护机制
Study on the protective effect of acupuncture on poor ovarian response in mice
摘要
Abstract
Objective To observe the protective effect of acupuncture on ovarian function and its impact on key molecules(Yes associated protein[YAP]and transcriptional coactivator with PDZ binding motif[TAZ])in the Hippo signaling pathway in poor ovarian response(POR)mice,in order to explore its mechanisms underlying improvement of POR by inhibiting ovarian cell apoptosis.Methods The mice with regular motility cycle after screening were randomly divided into normal control,model and acupuncture groups,with 12 mice in each group.The POR model was established by gavage of tripterygium wilfordii polyglycoside suspension(50 mg·kg-1·d-1)for 2 weeks,and the mice of the normal control group was received an equal volume of 0.9%sodium chloride solution by gavage.The mice in the acupuncture group received manual acupuncture stimulation of bilateral"Taichong"(LR3)and"Sanyinjiao"(SP6),and"Zhongji"(CV3)and"Guanyuan"(CV4),with the filiform needles retained for 20 min,once daily for successive 10 days.The estrous cycle was determined by using vaginal exfoliated cell smear,and the body mass was detected weekly.The levels of serum anti Mullerian hormone(AMH),follicle stimulating hormone(FSH),estradiol(E2)and luteinizing hormone(LH)were measured using enzyme-linked immunosorbent assay(ELISA).Histopathological changes of the ovarian tissue were observed after H.E.staining,and the apoptosis of ovarian granulosa cells was measured using terminal deoxynucleotidyl transferase(TdT)dUTP Nick-End Labeling(TUNEL)assay.The expression levels of YAP,TAZ,B-cell lymphoma-2(Bcl-2),Bcl-2 associated X(Bax),cysteine aspartic acid specific protease-3(Caspase-3)mRNAs and proteins were detected using real-time PCR and Western blot,respectively.Results Compared with the normal control group,the model group had an increase in the rate of estrous cycle disorder,estrous cycle,serum FSH and LH content,and apoptosis of ovarian granulosa cells,and expression levels of Bax and Caspase-3 mRNAs and p-YAP,Bax and Caspase-3 proteins(P<0.01),and a decrease in the body mass,number of retrieved oocytes,ovarian wet weight and ovarian index,serum AMH and E2 contents,and the expression levels of YAP,TAZ,Bcl-2 mRNAs and proteins(P<0.01).After acupuncture intervention,modeling induced increase and decrease of indexes mentioned above were completely reversed(P<0.05,P<0.01).H.E.staining showed deformed ovarian structure,reduced number of normal developing follicles and increased number of atretic follicles,disordered arrangement of the granulosa cells with fewer hierarchy in the model group,which was improved in the acupuncture group,such as increase in the number and improvement in the shape of normal ovarian follicles and reduction of the atretic follicles.Conclusion Acupuncture intervention can inhibit the apoptosis of ovarian granulosa cells and improve the ovarian function of POR mice,which may be related to its effects in up-regulating the expressions of YAP,TAZ(key molecules of Hippo signaling pathway).关键词
针刺/卵巢低反应/Hippo信号通路/细胞凋亡/性激素Key words
Acupuncture/Poor ovarian response/Hippo signal pathway/Apoptosis/Sex hormone引用本文复制引用
郝健亨,赵越梦,兰颖,刘璇,王海军,曹玉霞,冀来喜..针刺对卵巢低反应小鼠卵巢的保护机制[J].针刺研究,2024,49(10):1001-1009,9.基金项目
国家自然科学基金项目(No.82105028、82074549) （No.82105028、82074549）
