针刺研究2024，Vol.49Issue(10)：1056-1062,7.DOI:10.13702/j.1000-0607.20230341
艾灸通过调节滑膜G蛋白偶联受体43和辅助性T细胞17/调节性T细胞平衡改善佐剂性关节炎大鼠滑膜炎性反应
Effects of moxibustion in improving synovitis in adjuvant arthritis rats by regulating synovial GPR43 and peripheral blood Th17/Treg balance
摘要
Abstract
Objective To observe the effects of moxibustion on G protein-coupled receptor 43(GPR43)and helper T cell 17(Th17)/regulatory T cell(Treg)balance in rats with adjuvant arthritis(AA),so as to investigate the partial mechanism of moxibustion therapy on rheumatoid arthritis(RA).Methods A total of 24 Wistar rats were randomly divided into a normal group,a model group and a moxibustion group,with 8 rats in each group.The AA rat model was replicated using wind,cold and moisture environmental factors+Freund's complete adjuvant(CFA)injection method.In the moxibustion group,moxibustion was performed on bilateral"Zusanli"(ST36)and"Shenshu"(BL23)for 20 min/time,once daily,for 21 d.The changes of joint swelling degree(JSD)and arthritis index(AI)were observed in each group of rats.Transmission electron microscopy and HE staining were used to examine changes in the cellular structure of the ankle joint synovial tissue in each group.Western blot analysis was employed to detect the expression levels of GPR43 in the synovial tissue of the ankle joints.Flow cytometry was used to measure the percentages of Treg and Th17 in peripheral blood.ELISA was used to determine the contents of interleukin-10(IL-10)and transforming growth factor-β 1(TGF-β1)in serum.Results Compared with the normal group,the JSD and AI in the model group increased before and after treatment(P<0.01),with significant synovial pathological and ultrastruct ural injury observed.Additionally,compared with the normal group,the expression of GPR43 in the synovial tissue decreased(P<0.01),the Treg percentage in peripheral blood decreased(P<0.01)and the Th17 percentage increased(P<0.01),serum IL-10 and TGF-β1 contents decreased in the model group(P<0.01).Compared with the model group,the moxibustion group exhibited a significant reduction in JSD and AI after treatment(P<0.01),the degree of pathological and ultrastruct ural damage in the synovium decreased,the expression of GPR43 in the synovial tissue increased(P<0.05),the Treg percentage in peripheral blood increased(P<0.01)and the Th17 percentage decreased(P<0.01),serum IL-10 and TGF-β1 contents increased(P<0.01).The relative expression of GPR43 in synovial tissue was positively correlated with the percentage of Treg in peripheral blood(r=0.967,P<0.01).Conclusion Moxibustion can significantly improve the inflammatory response in the synovial membrane of AA rats.The mechanism may be related to moxibustion restoring the Th17/Treg balance through regulating GPR43.关键词
艾灸/G蛋白偶联受体43/辅助性T细胞17/调节性T细胞平衡/佐剂性关节炎Key words
Moxibustion/G protein-coupled receptor 43/Helper T cell 17/regulatory T cell balance/Adjuvant arthritis引用本文复制引用
霍晨露,刘安婷,李双,王淼,朱艳..艾灸通过调节滑膜G蛋白偶联受体43和辅助性T细胞17/调节性T细胞平衡改善佐剂性关节炎大鼠滑膜炎性反应[J].针刺研究,2024,49(10):1056-1062,7.基金项目
国家自然科学基金项目(No.81403484) （No.81403484）
安徽省高校自然科学基金项目(No.KJ2019A0448) （No.KJ2019A0448）
国家级项目培育基金项目计划(No.2019py01) （No.2019py01）
安徽省临床医学研究中心项目(No.安徽省科技厅皖社科[2020]41号) （No.安徽省科技厅皖社科[2020]41号）
安徽省中医药传承创新科研项目(No.2020ccyb13) （No.2020ccyb13）
