畜牧兽医杂志2024，Vol.43Issue(5)：80-82,3.DOI:10.7606/j.issn.1004-6704.2024.05.018
羊败血性链球菌多重PCR检测方法的建立
Establishment of a Multiplex PCR Method for Detection of Streptococcus Septicus in Sheep
摘要
Abstract
A multiplex PCR diagnostic method was es-tablished.The live vaccine of resurrected ovine Streptococcus septicus disease was used as the research object.According to the universal primers of bacterial 16S rRNA gene and the species-specific sodA gene of ovine streptococcus septicus,the primers were designed and synthesized and amplified by PCR.The results showed as follows:(1)The vaccine were in-oculated with BHI medium,the growth of bacteria was slow,and the BHI medium became cloudy after 24 h;On the blood plate,shiny,transparent,moist,viscous,smooth surface,neat edges,small dew drops,gray and white colonies were formed,and there was obvious beta hemolysis around the colonies.(2)The microscopic examination results of the bac-teria of the ovine septicemia streptococci vaccine strain showed that the microscopic examination showed gram stai-ning positive,often in pairs or 3 to 5 short chain arranged spherical or oval coccus,with capsules.(3)The results of nu-cleic acid extraction showed that the extracted nucleic acid was stable and could be used for subsequent experimental studies.(4)PCR amplification results showed that the method had good specificity and sensitivity,and could provide techni-cal support for laboratory diagnostic detection and molecular epidemiological investigation of sheep septicemia streptococ-cus disease.The study concluded that the multiplex PCR de-tection method is simple,rapid,sensitive and specific,easy to master,and generally suitable for grass-roots units to use,can quickly obtain diagnostic results,identify the pathogen,and pro-vide scientific basis for clinical rational drug use.关键词
羊/链球菌/多重聚合酶链反应/诊断Key words
sheep/streptococcus/multiplex PCR/diagno-sis分类
农业科技引用本文复制引用
魏梦君,郑思思,游潇倩,孙生祯,辛莉,阚威..羊败血性链球菌多重PCR检测方法的建立[J].畜牧兽医杂志,2024,43(5):80-82,3.基金项目
青海省高端创新人才千人计划项目 （）
