中国免疫学杂志2024，Vol.40Issue(10)：2031-2036,6.DOI:10.3969/j.issn.1000-484X.2024.10.002
十二烷基硫酸钠诱导的小鼠刺激性接触性皮炎及局部免疫微环境分析
Local immune microenvironment analysis of sodium dodecyl sulfate-induced irritant contact dermatitis model in mice
摘要
Abstract
Objective:To establish an irritant contact dermatitis(ICD)model induced by sodium dodecyl sulfate(SDS)in mice,and explore its endotype to provide an experimental and theoretical basis for subsequent precise treatment.Methods:Mice were randomly divided into two groups(model group and control group),4%SDS was topically applied to induce ICD in mice,saline was used on control group,the dose and frequency were consistent with model group,and the skin lesions of mice were observed.Epidermal thickness and inflammatory cell infiltration were analyzed by HE staining,toluidine blue staining and immunofluorescence staining.Real-time quantitative PCR was performed to investigate mRNA expression levels of cytokines.Results:Compared with control group,mice in ICD model group showed epidermal thickness on the back of neck,and the numbers of inflammatory cells were increased in dermis.The number of neutrophils,macrophages and T cells were increased.Expressions of Il17a and Il17f mRNA levels were increased.Conclusion:SDS-induced ICD model is successfully established,with the elevated infiltration of neutrophils,macrophages and T cells,and secretion of type 17 cytokines.关键词
刺激性接触性皮炎/内在型/中性粒细胞/17型炎症/白细胞介素17AKey words
Irritant contact dermatitis/Endotype/Neutrophil/Type 17 inflammation/IL-17A分类
生物科学引用本文复制引用
曾月玲,范立民,陈惠芳,何颖,陶爱林,刘雪婷..十二烷基硫酸钠诱导的小鼠刺激性接触性皮炎及局部免疫微环境分析[J].中国免疫学杂志,2024,40(10):2031-2036,6.基金项目
国家自然科学基金(82371797,82171764) （82371797,82171764）
广东省自然科学基金(2023A1515012484) （2023A1515012484）
广州市科信局市校(院)联合资助(高水平大学)基础研究项目(2024A03J0942) （院）
农业生物育种重大专项(2023ZD0406303) （2023ZD0406303）
广州市教育局产学研科研项目(14CXY03). （14CXY03）
