中国免疫学杂志2024，Vol.40Issue(10)：2037-2041,5.DOI:10.3969/j.issn.1000-484X.2024.10.003
苦精促进MrgprB2介导的鼠肥大细胞脱颗粒
Denatonium benzoate promotes MrgprB2-mediated rat mast cell degranulation
摘要
Abstract
Objective:To explore the potent effects of denatonium benzoate(DB)on Mas-related G protein-coupled receptor-B2(MrgprB2)-mediated rat mast cell degranulation.Methods:RBL-2H3 cells were treated with DB overnight,before challenged with MrgprB2 ligands substance P(SP).The release of β-hex from MrgprB2-activated RBL-2H3 was detected by substrate method.Detec-tion of LTC4,IL-6,TNF-α and cPLA2 activity were performed by ELISA.The Ca2+influx and the expression of RBL-2H3 MrgprB2 re-ceptors were measured by fluorescence assay.Results:The results showed 10 μmol/L,50 μmol/L,80 μmol/L,100 μmol/L DB treat-ments promoted β-hex and LTC4 releases from activated RBL-2H3,accompanied by increased Ca2+mobilization and cPLA2 activa-tion.DB treatments did not affect IL-6 and TNF-α LTC4 releases in MrgprB2-activated RBL-2H3,as well as the levels of MrgprB2 ex-pression in mast cells.Conclusion:Taken together,DB enhanced the MrgprB2-mediated RBL-2H3 degranulation in vitro,probably by up-regulating Ca2+mobilization in activated cells.关键词
苯甲地那铵/MrgprB2/肥大细胞脱颗粒/Ca2+活化Key words
Denatonium benzoate/MrgprB2/Mast cell degranulation/Ca2+mobilization分类
医药卫生引用本文复制引用
徐华平,石小云,邹节新,李欣,谢梦婷,肖诗宇,石林波..苦精促进MrgprB2介导的鼠肥大细胞脱颗粒[J].中国免疫学杂志,2024,40(10):2037-2041,5.基金项目
国家自然科学基金项目(21866020,32060306) （21866020,32060306）
江西省自然科学基金(20202BABL206108). （20202BABL206108）
