中国农业科学2024，Vol.57Issue(17)：3440-3457,中插24-中插33,28.DOI:10.3864/j.issn.0578-1752.2024.17.010
基于表型性状与SSR标记的冬瓜、节瓜种质资源遗传多样性分析
Genetic Diversity Analysis of Wax Gourd and Chieh-Qua Germplasm Resources Based on Phenotypic Traits and SSR Markers
摘要
Abstract
[Objective]To analyze the genetic diversity of wax gourd and chieh-qua germplasm resources,this study constructed a comprehensive evaluation system of wax gourd and chieh-qua germplasm and screened out excellent wax gourd and chieh-qua germplasms,so as to provide the valuable theoretical support for the innovation and variety selection of wax gourd and chieh-qua germplasm.[Method]148 wax gourd and chieh-qua germplasms from different sources were used as the test materials,and then the genetic diversity analysis was conducted using 28 phenotypic traits and 19 pairs of SSR markers.A variety of multiple statistical methods,including variation coefficient,Shannon-Wiener genetic diversity index,correlation analysis,principal component analysis,subordinate function value analysis,stepwise regression analysis,and cluster analysis,were used to analyze the genetic diversity and comprehensively evaluate on wax gourd and chieh-qua germplasm resources.[Result]Wax gourd and chieh-qua germplasm resources exhibited high genetic diversity,with the genetic diversity index of the 11 qualitative traits ranging from 0.39(Generotype)to 1.45(Fruit shape),the genetic diversity index of the 17 quantitative traits ranging from 1.89(First male flower node)to 2.09(Petiole length),and the variation coefficients of the 17 quantitative traits ranging from 9.76%(Seed shape index)to 63.95%(Weight per fruit).The variation coefficients of the first female flower node(42.32%),fruit length(42.95%),fruit shape index(47.05%),first male flower node(47.48%)and weight per fruit(63.95%)were all greater than 40%,which showed great potential for genetic improvement.Principal component analysis showed that the 18 main phenotypic traits were integrated into 6 principal components,with contribution rates ranging from 6.427%to 29.605%,and the additive contributing rate came up to 81.236%.The phenotypic comprehensive evaluation F value calculated by subordinate function values analysis showed that the BR12(1.47)and BR25(1.14)had the best characteristics.15 phenotypic traits were extremely significantly correlated with the F value.The mathematical model of phenotypic evaluation was established by stepwise regression analysis,and 9 phenotypic comprehensive evaluation indicators were screened out.The number of alleles amplified by 19 SSR primers in the population material ranged from 2 to 6,the variation range of polymorphic information was 0.02-0.70,the Shannon's diversity index distribution range was 0.06-1.48,which showed that 148 wax gourd and chieh-qua germplasm resources had rich genetic diversity.The genetic diversity of chieh-qua population was slightly higher than that of wax gourd population,and the genetic variation within individuals was the main reason for the overall genetic diversity of wax gourd and chieh-qua.Based on the classification of phenotypic traits and molecular markers,148 wax gourd and chieh-qua germplasm resources were divided into 6 categories and 3 categories,respectively.The clustering results of the two methods did not cluster wax gourd and chieh-qua resources into two categories,and there was no obvious correlation with geographical location.[Conclusion]148 wax gourd and chieh-qua germplasms resources had rich phenotypic genetic variation and high genetic diversity.The leaf length,first female flower node,fruit length,fruit thick,pedicle length,seed width,wax powder,fruit shape and seed type could be used as the key indicators for identifying wax gourd and chieh-qua germplasm resources.The clustering results based on the classification of main agronomic traits and molecular markers were consistent to some extent.There was no obvious correlation between the cluster and the geographical origin.关键词
冬瓜/节瓜/表型性状/SSR标记/遗传多样性/综合评价Key words
wax gourd/chieh-qua/phenotypic traits/SSR markers/genetic diversity/comprehensive evaluation引用本文复制引用
翟彩娇,葛礼姣,程玉静,仇亮,王小秋,刘水东..基于表型性状与SSR标记的冬瓜、节瓜种质资源遗传多样性分析[J].中国农业科学,2024,57(17):3440-3457,中插24-中插33,28.基金项目
江苏省省级农业科技创新与推广补助专项(南通特色蔬菜志)、南通市科技创新智库项目(CXZK202304)、江苏沿江地区农业科学研究所学科建设基金(YJXK[2021]101) （南通特色蔬菜志）
